Application and exploring of the induction condition of secondary metabolites producing genes in Rhodococcus jostii RHA1

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Rhodococcus jostii RHA1 was grown on a broad range of aromatic compounds and vigorously degrades environmental pollutants, such as polychlorinated biphenyls (PCBs). Based on the genome analysis of RHA1, many secondary metabolites genes including polyketide synthase (PKS) and non-ribosomal dependent peptide synthase (NRPS) were found on chromosome and plasmids, as same as other actinomycetes. In this study, induction conditions of these genes were investigated to understand what isa role of secondary metabolites from these genes on particular conditions. Type I, type III, PKS-NRPS hybrid, and NRPSs on plasmid were mainly induced on solid medium. On the other hands, type II PKSs were induced on liquid medium. For understanding of the secondary metabolism in actinomycetes, we are now trying to analyze chemical composition of these secondary metabolites.