Application and exploring of the induction condition of secondary metabolites producing genes in Rhodococcus jostii RHA1

红球菌RHA1次级代谢产物产生基因诱导条件的应用与探索

• 批准号: 22658029
• 负责人: HARA Hirofumi
• 金额: $ 2.39万
• 依托单位: Okayama University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658029/
• 关键词: 放線菌; ロドコッカス属; ゲノム; 抗生物質生産; 転写誘導

Rhodococcus jostii RHA1 was grown on a broad range of aromatic compounds and vigorously degrades environmental pollutants, such as polychlorinated biphenyls (PCBs). Based on the genome analysis of RHA1, many secondary metabolites genes including polyketide synthase (PKS) and non-ribosomal dependent peptide synthase (NRPS) were found on chromosome and plasmids, as same as other actinomycetes. In this study, induction conditions of these genes were investigated to understand what isa role of secondary metabolites from these genes on particular conditions. Type I, type III, PKS-NRPS hybrid, and NRPSs on plasmid were mainly induced on solid medium. On the other hands, type II PKSs were induced on liquid medium. For understanding of the secondary metabolism in actinomycetes, we are now trying to analyze chemical composition of these secondary metabolites.

乔氏红球菌（Rhodococcus jostii） RHA1生长在多种芳香族化合物上，对多氯联苯（PCBs）等环境污染物具有很强的降解能力。基于对RHA1基因组的分析，与其他放线菌一样，在染色体和质粒上发现了包括聚酮合成酶（PKS）和非核糖体依赖肽合成酶（NRPS）在内的许多次级代谢产物基因。在本研究中，研究了这些基因的诱导条件，以了解这些基因的次生代谢物在特定条件下的作用。I型、III型、PKS-NRPS杂交种和质粒上的NRPSs主要在固体培养基上诱导。另一方面，II型PKSs在液体培养基上诱导。为了更好地了解放线菌的次生代谢，我们正在尝试分析这些次生代谢产物的化学成分。

项目成果

ロドコッカス属細菌における二次代謝産物生合成遺伝子群の誘導条件の探索

寻找红球菌次级代谢产物生物合成基因的诱导条件

• 作者: Nagata;Y.;et al.;永田裕二;川角徹ら;原 啓文
• 通讯作者: 原 啓文