Genome wide localization analysis of BphT1 binding region on Rhodococcus jostii RHA1.

点击翻译按钮获取中文摘要

Rhodococcus jostii RHA1 was grown on a broad range of aromatic compounds and vigorously degrades polychlorinated biphenyls (PCBs). Two-component signal transduction system, BphT1T2, is necessary for activation of biphenyl degradation genes in R. jostii RHA1. In this study, we performed global chromatin immunopercipitation-on-chip (ChIP-chip) analysis to identify novel BphT1 binding region in R. jostii RHA1. As a result, we identified 9 ORFs as direct target of BphS1T1, which including IclR type transcriptional regulator, possible endonuclease, and some hypothetical proteins. This result indicated that BphS1T1 is a master regulator of biphenyl degradation, and directly activated to transcriptional regulator and endonibonuclease to regulate expression level of many ORFs in RHA1.