Investigation of Chaperone Function of Phospholipids Containing Polyunsaturated Fatty Acids and Their Application to Overproduction of Membrane Proteins
Investigation of Chaperone Function of Phospholipids Containing Polyunsaturated Fatty Acids and Their Application to Overproduction of Membrane Proteins
批准号:
22658028
负责人:
KURIHARA Tatsuo
金额:
$2.28万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
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英文摘要
Shewanella livingstonensis Ac10 produces eicosapentaenoic acid-containing phospholipids (EPA-PLs) at low temperatures. We analyzed the role of EPA-PLs in the folding of Omp74, a major cold-inducible outer membrane protein of this bacterium. We found that the conformation of Omp74 in the mutant lacking EPA-PLs is different from that in the wild-type strain. In vitro experiments using liposomes showed that EPA-PLs function as a chemical chaperone in the membrane integration and folding of Omp74.
期刊论文(0)
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科研奖励(0)
会议论文
Molecular probes to analyze the dynamics of eicosapentaenoic-acid-containing phospholipids and their interaction with membrane proteins
分子探针分析含二十碳五烯酸磷脂的动力学及其与膜蛋白的相互作用
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Nagata, Y, et aL, 栗原達夫]
通讯作者:
栗原達夫
Function of eicosapentaenoic acid-containing phospholipids in bacterial cell membrane
含二十碳五烯酸的磷脂在细菌细胞膜中的功能
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Kurihara T]
通讯作者:
Kurihara T
Diversity of acyl groups of phospholipids in bacterial cell membranes: Its generation mechanism and physiological significance
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批准号:18H02127
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.15万
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财政年份:2018
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负责人:KURIHARA Tatsuo
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依托单位:
Elucidation of the mechanism of extracellular membrane vesicle production with a novel bacterium that abundantly produces vesicles and their application
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批准号:18K19178
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项目类别:Grant-in-Aid for Challenging Research (Exploratory)
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资助金额:$3.99万
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财政年份:2018
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负责人:KURIHARA Tatsuo
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依托单位:
Exploration and development of cold-adapted microorganisms useful in low-temperature biotechnology for chemical production and environmental conservation
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批准号:17H04598
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.15万
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财政年份:2017
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负责人:KURIHARA Tatsuo
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依托单位:
Analysis of Cold-Adaptation Mechanism of Food Spoilage Bacteria and Its Application to Food Industry
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批准号:15K14732
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.5万
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财政年份:2015
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负责人:KURIHARA Tatsuo
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依托单位:
Mechanism of Biogenesis of Membrane Microdomain Containing Polyunsaturated Fatty Acids in Bacteria and Its Physiological Functions
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批准号:15H04474
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.9万
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财政年份:2015
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负责人:KURIHARA Tatsuo
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依托单位:
Exploration of Cold-Adapted Microorganisms to Develop Low-Temperature Bioprocessing Systems
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批准号:25303028
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.65万
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财政年份:2013
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负责人:KURIHARA Tatsuo
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依托单位:
Development of Biocatalysts for Remediation of Environments Polluted with Persistent Organohalogen Compounds
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批准号:24658275
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项目类别:Grant-in-Aid for Challenging Exploratory Research
-
资助金额:$2.58万
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财政年份:2012
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负责人:KURIHARA Tatsuo
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依托单位:
Functional analysis and application of phospholipids containing polyunsaturated fatty acids in bacterial cell membrane
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批准号:24380047
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.56万
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财政年份:2012
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负责人:KURIHARA Tatsuo
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依托单位:
Exploration of cold-adapted microorganisms for development of new low-temperature biotechnological processes
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批准号:22404021
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.82万
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财政年份:2010
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负责人:KURIHARA Tatsuo
-
依托单位:
Analysis of the molecular basis for cold adaptation of psychrotrophic bacteria
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批准号:20360372
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.9万
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财政年份:2008
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负责人:KURIHARA Tatsuo
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依托单位:
Exploration of novel cold-adapted microorganisms to develop a systemfor the production of useful compounds at low temperatures
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批准号:19404020
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.9万
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财政年份:2007
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负责人:KURIHARA Tatsuo
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依托单位:
Conversion of Organofluorine Compounds with Microbial Enzymes : Mechanistic Analysis of the Enzyme Reactions and Their Application to Production of Useful Compounds and Bioremediation of Environments
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批准号:17360397
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.78万
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财政年份:2005
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负责人:KURIHARA Tatsuo
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依托单位:
Exploration of Novel Cold-adapted Microorganisms That Inhabit the Polar Regions and Investigation of Their Useful Enzymes
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批准号:17404021
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项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$8.26万
-
财政年份:2005
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负责人:KURIHARA Tatsuo
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依托单位:
海外基金