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Investigation of Chaperone Function of Phospholipids Containing Polyunsaturated Fatty Acids and Their Application to Overproduction of Membrane Proteins

Investigation of Chaperone Function of Phospholipids Containing Polyunsaturated Fatty Acids and Their Application to Overproduction of Membrane Proteins
含多不饱和脂肪酸磷脂的伴侣功能研究及其在膜蛋白过量生产中的应用
批准号:
22658028
负责人:
KURIHARA Tatsuo
金额:
$2.28万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011

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中文摘要
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英文摘要
Shewanella livingstonensis Ac10 produces eicosapentaenoic acid-containing phospholipids (EPA-PLs) at low temperatures. We analyzed the role of EPA-PLs in the folding of Omp74, a major cold-inducible outer membrane protein of this bacterium. We found that the conformation of Omp74 in the mutant lacking EPA-PLs is different from that in the wild-type strain. In vitro experiments using liposomes showed that EPA-PLs function as a chemical chaperone in the membrane integration and folding of Omp74.
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Molecular probes to analyze the dynamics of eicosapentaenoic-acid-containing phospholipids and their interaction with membrane proteins
分子探针分析含二十碳五烯酸磷脂的动力学及其与膜蛋白的相互作用
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [Nagata, Y, et aL, 栗原達夫]
通讯作者: 栗原達夫
Function of eicosapentaenoic acid-containing phospholipids in bacterial cell membrane
含二十碳五烯酸的磷脂在细菌细胞膜中的功能
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [Kurihara T]
通讯作者: Kurihara T
Diversity of acyl groups of phospholipids in bacterial cell membranes: Its generation mechanism and physiological significance
  • 批准号:
    18H02127
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.15万
  • 财政年份:
    2018
  • 负责人:
    KURIHARA Tatsuo
  • 依托单位:
Elucidation of the mechanism of extracellular membrane vesicle production with a novel bacterium that abundantly produces vesicles and their application
  • 批准号:
    18K19178
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
  • 资助金额:
    $3.99万
  • 财政年份:
    2018
  • 负责人:
    KURIHARA Tatsuo
  • 依托单位:
Exploration and development of cold-adapted microorganisms useful in low-temperature biotechnology for chemical production and environmental conservation
  • 批准号:
    17H04598
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.15万
  • 财政年份:
    2017
  • 负责人:
    KURIHARA Tatsuo
  • 依托单位:
Analysis of Cold-Adaptation Mechanism of Food Spoilage Bacteria and Its Application to Food Industry
  • 批准号:
    15K14732
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.5万
  • 财政年份:
    2015
  • 负责人:
    KURIHARA Tatsuo
  • 依托单位:
海外基金