Exploration of cold-adapted microorganisms for development of new low-temperature biotechnological processes

探索适应寒冷的微生物以开发新的低温生物技术工艺

• 批准号: 22404021
• 负责人: KURIHARA Tatsuo
• 金额: $ 10.82万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22404021/
• 关键词: 特殊環境微生物; 低温菌; 好冷性酵素; 分子育種; 国際研究者交流; 国際情報交換; オーストリア; メタル代謝; 高度不飽和脂肪酸; ドイツ; 生物・生体工学; 低温適応微生物

New cold-adapted microorganisms were isolated from cold environments, and biochemical and biotechnological studies on cold-adapted microorganisms were performed for development of new low-temperature bioprocesses. In particular, a genetic engineering method to modify the genome of Shewanella livingstonensis Ac10, a cold-adapted bacterium isolated from Antarctic seawater, was established for its molecular breeding. Eicosapentaenoic acid, which is beneficial to human health, was found to be produced by this strain, and the mechanism of its biosynthesis was elucidated. In addition, the strain was shown to reduce various metal ions, and a protein involved in this process was identified for metal biotechnological application of this strain.

从低温环境中分离出新的冷适应微生物，并对冷适应微生物进行生化和生物技术研究，以开发新的低温生物工艺。特别是建立了从南极海水中分离到的一株耐冷细菌希瓦氏菌（Shewanella livingstonensis Ac10）基因组改造的基因工程方法，为希瓦氏菌的分子育种奠定了基础。发现该菌株能产生对人体有益的二十碳五烯酸，并阐明了其生物合成机制。此外，该菌株被证明可以还原各种金属离子，并鉴定了参与该过程的蛋白质，以用于该菌株的金属生物技术应用。

项目成果

Functional analysis of an eicosapentaenoic acid biosynthesis protein Orf2 from a psychrotrophic bacterium, Shewanella livingstonensis Ac10

来自耐冷细菌 Shewanella Livingstonensis Ac10 的二十碳五烯酸生物合成蛋白 Orf2 的功能分析

• 发表时间: 2012
• 期刊: Trace Nutrients Research
• 作者: Chunjie GONG;Jun KAWAMOTO;Nobuyoshi ESAKI;and Tatsuo KURIHARA
• 通讯作者: and Tatsuo KURIHARA

低温菌 Shewanella livingstonensis Ac10 による金属還元機構の解析

耐冷菌Shewanella Livingstonensis Ac10金属还原机制分析

• 发表时间: 2013
• 作者: 丸山沙織;樽井淳;王玉;川本純;栗原達夫
• 通讯作者: 栗原達夫

低温菌Shewanella livingstonensis Ac10による金属還元機構の解析

耐冷菌希瓦氏菌Ac10还原金属机制分析

• 发表时间: 2013
• 作者: 丸山沙織;樽井淳;王玉;川本純;栗原達夫
• 通讯作者: 栗原達夫

Function of phospholipids containing eicosapentaenoic acid in the bacterial cell membrane

含二十碳五烯酸的磷脂在细菌细胞膜中的功能

• 发表时间: 2012
• 作者: Wang Q.;Chen Q.;P. Apaer;N.Kashiwagi;H. Kurokawa;K. Sugiyama;X. Wang and X. Guo;Tatsuo KURIHARA
• 通讯作者: Tatsuo KURIHARA

Characterization of 1-acyl-sn-glycerol-3-phosphate acyltransferase from a polyunsaturated fatty acid-producing bacterium, Shewanella livingstonensis Ac10

来自多不饱和脂肪酸生产菌 Shewanella Livingstonensis Ac10 的 1-酰基-sn-甘油-3-磷酸酰基转移酶的表征

• 发表时间: 2012
• 期刊: Trace Nutrients Research
• 作者: Hyun-Nam CHO;Wataru KASAI;Jun KAWAMOTO;Nobuyoshi ESAKI;and Tatsuo KURIHARA
• 通讯作者: and Tatsuo KURIHARA