Development of long-term protein expression for medical application of gene delivery technology and its therapeutic application
Development of long-term protein expression for medical application of gene delivery technology and its therapeutic application
批准号:
22659110
负责人:
KAWAKAMI Shigeru
金额:
$1.96万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
点击翻译按钮获取中文摘要
英文摘要
In vivo gene transfection technology is an important method for the establishment of gene therapy, gene functional analysis, and diseased animal model. In this study, we have succeeded to develop a long-term gene transfection method that is induced by integration of specific sites in the genome and tissue pressure and/or suction-mediated gene transfection methods for naked plasmid DNA. This information would be efficient for the establishment of new therapeutic strategies for refractory diseases.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
Evaluation of long-term gene expression in mouse liver using phiC31 integrase and hydrodynamic injection
使用 phiC31 整合酶和流体动力学注射评估小鼠肝脏中的长期基因表达
DOI:
--
发表时间:
2012
期刊:
Biological and Pharmaceutial Bulletin
影响因子:
--
作者:
[Y.Umemoto, S.Kawakami, Y.Otani, Y.Higuchi, F.Yamashita, M.Hashida]
通讯作者:
M.Hashida
臓器特異的遺伝子発現制御を目的とする組織押圧核酸導入法の開発と評価
用于器官特异性基因表达控制的组织压力核酸递送方法的开发和评估
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[向井英史, 川上茂, 橋田充]
通讯作者:
橋田充
φC31 integraseによるin vivo長期遺伝子発現の簡便な評価を目的とした分泌型luciferase発現pDNAの構築
构建表达分泌型荧光素酶的 pDNA,用于通过 φC31 整合酶简单评估体内长期基因表达
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[梅本佳昭, 川上茂, 大谷祐基, 山下富義, 橋田充]
通讯作者:
橋田充
phiC31 integrase-mediated long-term gene expression in the cultured renal cell lines and murine kidney
培养肾细胞系和小鼠肾脏中phiC31整合酶介导的长期基因表达
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Shigeru Kawakami, Yuki Otani, Hidefumi Mukai, Yoshiaki Umemoto, Fumiyoshi Yamashita, and Mitsuru Hashida]
通讯作者:
and Mitsuru Hashida
DOI:
10.1248/bpb.33.1627
发表时间:
2010-09-01
期刊:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
影响因子:
2
作者:
[Mukai, Hidefumi, Kawakami, Shigeru, Hashida, Mitsuru]
通讯作者:
Hashida, Mitsuru
共 10 条
Elucidation of mechano-transfection and mechano-sensing mechanism using a living tissue
-
批准号:17K19496
-
项目类别:Grant-in-Aid for Challenging Research (Exploratory)
-
资助金额:$4.16万
-
财政年份:2017
-
负责人:KAWAKAMI Shigeru
-
依托单位:
Development of gene delivery system using the tissue press/suction method
-
批准号:26670082
-
项目类别:Grant-in-Aid for Challenging Exploratory Research
-
资助金额:$2.41万
-
财政年份:2014
-
负责人:KAWAKAMI Shigeru
-
依托单位:
Development of tumor associated macrophages-selective DDS and its application to tumor therapy
-
批准号:23689003
-
项目类别:Grant-in-Aid for Young Scientists (A)
-
资助金额:$17.8万
-
财政年份:2011
-
负责人:KAWAKAMI Shigeru
-
依托单位:
Development of efficient gene transfection system by the combination use of dendritic cells-selective nanoparticle and physical stimulation for cancer therapy
-
批准号:19689005
-
项目类别:Grant-in-Aid for Young Scientists (A)
-
资助金额:$15.14万
-
财政年份:2007
-
负责人:KAWAKAMI Shigeru
-
依托单位:
海外基金