Dual regulation of secretion system of periodontal virulence factors by novel two-component system and ECF sigma factor

新型双组分系统和ECF西格玛因子对牙周毒力因子分泌系统的双重调节

• 批准号: 22659332
• 负责人: NAKAYAMA Koji
• 金额: $ 2.01万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659332/
• 关键词: 遺伝子; 発現調節; 二成分制御系; シグマ因子; 歯周病; 歯学; 細菌; プロテアーゼ; 膜輸送

We investigated regulatory mechanism of secretion system of pathogenic proteases/ adhesins of the periodontopathogen Porphyromonas gingivalis. Secretion of P. gingivalis gingipain proteases/ adhesins depends on a protein secretion system, Por secretion system(PorSS). We isolated 12 gingipain secretion-deficient mutants and found that two of them were deficient in a two-component system, which is a prokaryotic signaling system and consists of the sensor histidine kinase PorY and response regulator PorX. In this study, we constructed recombinant PorY and PorX proteins and found that PorY was autophosphorylated and the phosphoryl residue of PorY was transferred to PorX. PorY could bind to PorX as revealed by Surface plasmon resonance(BIACORE) analysis. Gene expression of component proteins of the PorSS was also regulated by one of ECF sigma factors, PGN0274, but the PorY-PorX system and the PGN0274 sigma factor independently regulate gene expression of component proteins of the PorSS, suggesting that the PorSS is regulated by multiple regulatory mechanisms.

我们研究了牙周病原菌牙龈卟啉单胞菌致病性蛋白酶/粘附素分泌系统的调节机制。牙龈卟啉单胞菌牙龈蛋白酶/粘附素的分泌依赖于蛋白质分泌系统，Por分泌系统(PorSS)。我们分离了 12 个牙龈蛋白酶分泌缺陷突变体，发现其中两个缺乏双组分系统，该系统是原核信号系统，由传感器组氨酸激酶 PorY 和反应调节器 PorX 组成。在本研究中，我们构建了重组PorY和PorX蛋白，发现PorY被自磷酸化，并且PorY的磷酰基残基被转移到PorX上。表面等离子共振 (BIACORE) 分析表明，PorY 可以与 PorX 结合。 PorSS的组成蛋白的基因表达也受到ECF sigma因子之一PGN0274的调节，但PorY-PorX系统和PGN0274 sigma因子独立调节PorSS的组成蛋白的基因表达，表明PorSS受到多种调节机制的调节。

项目成果

比較ゲノム解析から発見されたグラム陰性細菌の新規タンパク分泌機構

通过比较基因组分析发现革兰氏阴性菌的新蛋白质分泌机制

• 发表时间: 2010
• 作者: Kaneda M;Zhang D;Bhattacharjee R;Nakahama KI;Arii S;Morita I.;中山浩次
• 通讯作者: 中山浩次

Hemmaglutinin/adhesion Domains of Porphyromonas gingivalis Play Key Roles in Coaggregation with Treponema denticola

牙龈卟啉单胞菌的血凝素/粘附结构域在与齿垢密螺旋体的共聚中发挥关键作用

• 发表时间: 2010
• 期刊: FEMS Immunol.Med.Microbiol.
• 作者: Ito R;Ishihara K;Shoji M;Nakayama K;Okuda K
• 通讯作者: Okuda K

Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis: its cellular distribution, thioredoxin activity and role in heme utilization.

• DOI: 10.1186/1471-2180-10-152
• 发表时间: 2010-05-25
• 期刊: BMC microbiology
• 影响因子: 4.2
• 作者: Shoji M;Shibata Y;Shiroza T;Yukitake H;Peng B;Chen YY;Sato K;Naito M;Abiko Y;Reynolds EC;Nakayama K
• 通讯作者: Nakayama K

Tetratricopeptide Repeat Protein-Associated Proteins Contribute to the Virulence of Porphyromonas gingivalis

• DOI: 10.1128/iai.01448-09
• 发表时间: 2010-06-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Kondo, Yoshio;Ohara, Naoya;Nakayama, Koji
• 通讯作者: Nakayama, Koji

Genome analysis and a novel secretion system of the periodontal pathogen Porphyromonas gingivalis.

牙周病原体牙龈卟啉单胞菌的基因组分析和新型分泌系统。

• 发表时间: 2010
• 作者: 周防加奈、谷口文紀、原田省寺川直樹;他;中山浩次
• 通讯作者: 中山浩次