Molecular dissection of membrane traffic machinery regulating preterm delivery induced by marginal periodontitis

人类的流行病学和干预性研究揭示了牙周病与低出生体重儿早产之间的密切联系。牙龈卟啉单胞菌是一种牙周致病菌，经口腔-血源性传播后可转移至妊娠组织。我们以前表明，牙龈卟啉单胞菌侵入绒毛外滋养层细胞（HTR-8）来自人胎盘，并通过诱导细胞周期的G1期阻滞抑制增殖。本研究的目的是确定介导牙龈卟啉单胞菌引起的细胞损伤的信号通路。结果提示牙龈卟啉单胞菌激活细胞DNA损伤信号通路，导致滋养层细胞G1期阻滞和凋亡。

Epidemiological and interventional studies of humans have revealed a close association between periodontal diseases and preterm delivery of low birth weight infants. Porphyromonas gingivalis, a periodontal pathogen, can translocate to gestational tissues following oral-hematogenous spread. We previously showed that P. gingivalis invades extravillous trophoblast cells(HTR-8) derived from the human placenta, and inhibits proliferation through induction of arrest in the G1 phase of the cell cycle. The purpose of the present study was to identify signaling pathways mediating cellular impairment caused by P. gingivalis. The obtained results results suggest that P. gingivalis gingivalis activates cellular DNA damage signaling pathways that lead to G1 arrest and apoptosis in trophoblasts.