PIPs and Ca2+-dependent regulation on Ca channels

点击翻译按钮获取中文摘要

TRPC3/C6/C7 channels, a subgroup of classical/canonical TRP channels, are activated by diacylglycerol produced via activation of phospholipase C (PLC)-coupled receptors. The critical importance of PIPs to various ion-transporting molecules is well documented, but their function in relation to these channels remains controversial. We used voltage-sensing phosphatase (DrVSP) to study TRPC3/C6/C7 regulation and found that the channel activity was controlled by PIP2-DAG signaling in a self-limiting manner. We also simulated the channel dynamics based on this self-limiting hypothesis. Overall, our studies demonstrate a fundamental regulatory mechanism of TRPC3/6/7 activation by PIP2-DAG signal.