Development of metal probes for the selective recognition of DNA

开发用于选择性识别 DNA 的金属探针

• 批准号: LX0345638
• 负责人: Prof Peter Karuso
• 金额: $ 5.07万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage - International
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2004-02-28
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LX0345638
• 关键词: Development; metal; probes; selective; recognition

Transition metal complexes with large aromatic ligands have recently been shown to bind DNA by intercalation. However, controversy continues over their base sequence and groove selectivity. We will synthesise a range of chiral metal complexes and screen these against combinatorial oligonucleotide libraries to determine their base sequence selectivity. The information obtained will help us design better intercalators that can be used as sensitive molecular probes and may find application as antitumour drugs. Binding interaction between the complex and DNA will be studied by NMR spectroscopy.

最近已证明具有大芳香族配体的过渡金属配合物可以通过嵌入结合 DNA。然而，关于它们的碱基序列和凹槽选择性的争议仍在继续。我们将合成一系列手性金属配合物，并针对组合寡核苷酸文库进行筛选，以确定它们的碱基序列选择性。获得的信息将帮助我们设计更好的嵌入剂，可用作敏感的分子探针，并可能作为抗肿瘤药物得到应用。 复合物与 DNA 之间的结合相互作用将通过 NMR 光谱进行研究。