Three-dimensional stress strain measurement by micro/nano mechanical test under second harmonic generation microscope

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We developed a second harmonic generation(SHG) microscope technic for non-contact and low-invasion observation of internal stress of collagen fibers, which is main components of extracellular matrix, and gives mechanical strength to soft tissue like skin. We demonstrated the potential of the technic by showing that the intensity of SHG light from collagen fibers had a dependence on the internal stress induced by tensile tester on SHG microscope, which focus ultra-short pulsed laser on collagen fibers.