The role of Rho-mDia siganling in tissue architecture and homeostasis in the body

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Here we examined action and regulation of mDia, a Rho-regulated actin nucleator/polymerization factor, at the molecular and cellular level and the role of each mDia isoform in tissue architecture and maintenance in the body. In the former, we discovered that mDia catalyzes actin polymerization by rotating along the pitch of actin filament and that Liprin-alpha negatively regulating mDia activity by trapping mDia in the cytosol. In the latter, we used mDia-deficient KO mice and discovered that mDia contribute to shaping and maintenance of architecture of the brain by regulating the neuroepithelial polarity, tangential migration of inhibitory neuron precursors and axon guidance of the corticospinal tract neurons. We also discovered that mDia2 regulates cytokinesis of erythroblasts and contributes to maintenace of the hematopoietic system. In this work, we further elucidated how mDia-induced actin cytoskeleton functions in each of these in vivo processes.