The role of Rho-mDia siganling in tissue architecture and homeostasis in the body

在这里，我们研究了mdia的作用和调控，mdia是一种受Rho调节的肌动蛋白核因子/聚合因子，在分子和细胞水平上，以及每种mdia亚型在组织结构和体内维持中的作用。在前者中，我们发现MDIA通过沿肌动蛋白细丝的节距旋转来催化肌动蛋白聚合，而Liprin-α通过将MDIA捕获在细胞质中来负向调节MDIA的活性。在后者中，我们使用了MDIA缺陷的KO小鼠，发现MDIA通过调节神经上皮极性、抑制性神经元前体的切向迁移和皮质脊髓束神经元的轴突引导，有助于脑结构的形成和维持。我们还发现，mDia2调节红细胞的胞质分裂，有助于维持造血系统。在这项工作中，我们进一步阐明了MDIA诱导的肌动蛋白细胞骨架如何在体内这些过程中发挥作用。

Here we examined action and regulation of mDia, a Rho-regulated actin nucleator/polymerization factor, at the molecular and cellular level and the role of each mDia isoform in tissue architecture and maintenance in the body. In the former, we discovered that mDia catalyzes actin polymerization by rotating along the pitch of actin filament and that Liprin-alpha negatively regulating mDia activity by trapping mDia in the cytosol. In the latter, we used mDia-deficient KO mice and discovered that mDia contribute to shaping and maintenance of architecture of the brain by regulating the neuroepithelial polarity, tangential migration of inhibitory neuron precursors and axon guidance of the corticospinal tract neurons. We also discovered that mDia2 regulates cytokinesis of erythroblasts and contributes to maintenace of the hematopoietic system. In this work, we further elucidated how mDia-induced actin cytoskeleton functions in each of these in vivo processes.