Design of nanostructured carbon film electrode for developing drug metabolizing screening chip

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Electrochemical biosensors consisting of cytochrome P450 modified electrodes have been developed for screening the metabolism of a drug and its inhibitor by using nanostructured carbon materials. First, we developed nanostructured carbon film electrode by UV/ozone treatment of sputtered carbon film. This electrode is suitable for efficient direct electron transfer with small enzymes such as bilirubin oxidase but not good for cytochrome P450(CYP) which is well known drug metabolism enzyme. Then, carbon nanofiber(CNF) modified and CYP modified electrode was applied for drug metabolism and inhibitor screening. DET between CYP and CNF was observed at -0.302V. The electrocatalytic reduction current increased with the addition of drugs. In contrast, the current was greatly reduced in the presence of inhibitor. Recently, we found that polycrystalline ITO electrode with rough surface structure is suitable as drug metabolism and inhibitor screening sensor even with CYP microsome.