Development of highly electroactive nitrogen-doped graphene like film electrode for nonlabel biosennsing applications

采用电子回旋共振（ECR）溅射法制备氮掺杂纳米碳膜电极。在中等氮浓度下，ECR溅射的CN膜呈现出增强的类富勒烯微观结构。这种结构由弯曲和交联的石墨烯片组成，随着sp3键的增加，层状面积显著减少。该薄膜具有非常光滑的表面，平均粗糙度为0.1至0.2 nm。这些化学结构的变化极大地改变了电化学性能。由于其较高的sp3含量，N-ECR电极显示出更宽的电位窗口。N- ecr电极（N = 9.0 at）。%)表现出提高的电化学活性。N-ECR电极上的氧还原电位和过氧化氢还原电位分别移动了约0.3和0.15 V， H2O2的峰高大大增加。N-ECR电极对鸟苷和腺苷等生物分子也表现出较好的氧化活性。

A nitrogen-doped nanocarbon film electrode formed using the electron cyclotron resonance (ECR) sputtering method.The ECR sputtered CN films exhibited an enhanced fullerene-like microstructure at intermediate nitrogen concentration. This structure consisted of bent and cross-linked graphene sheets where layered areas remarkably decreased with increasing sp3 bonding. The film has a very smooth surface with an average roughness of 0.1 to 0.2 nm. These variations in the chemical structures greatly change the electrochemical performance. The N-ECR electrode shows a wider potential window due to its higher sp3 content. The N-ECR electrode (N = 9.0 at. %) shows improved electrochemical activity. The oxygen and hydrogen peroxide reduction potentials at the N-ECR electrode shifted about 0.3 and 0.15 V, respectively, and the peak height of H2O2 is greatly increased.The N-ECR electrode also shows better activity for oxidizing certain biomolecules such as guanosine and adenosine decreased.