Precise Characterization of Macromolecular Assemblies for Quality Control of Biomaterials

大分子组件被用作药物递送系统、纳米载体或生物传感器等生物材料。大分子组装体的精确表征对于控制这些生物材料的质量至关重要。在这项研究中，我们建立了表征溶液中热敏嵌段共聚物和多电解质配合物的方法。所研究的三种热敏嵌段共聚物均发生了同时胶束化和相分离，均为弱分离的弱两亲性嵌段共聚物。用小角x射线散射对溶液中的胶束和浓相液滴进行了表征。采用静态光散射法和电泳光散射法测定了水溶液中两种聚阴离子和两种聚阳离子的四种组合的聚电解质复合物的净电荷数和总电荷数（或聚集数），并与所提出的复合物形成机制进行了比较。

Macromolecular assemblies are utilized as biomaterials like drug delivery system, nano-carrier, or bio-sensor. Precise characterization of macromolecular assemblies is essential to control the quality of those biomaterials. In this study, we have established the method for characterizing assemblies of thermoresponsive block copolymers and polyelectrolyte complexes in solution. Simultaneous micellization and phase separation took place in three thermoresponsive block copolymers investigated, all of which were weakly segregated and weakly amphiphilic block copolymers. Both micelles and concentrated-phase droplets in the solutions were characterized by small-angle X-ray scattering. The number of net charges and total charges (or the aggregation number) per the polyelectrolyte complex were determined by static and electrophoretic light scattering methods for four combinations of two polyanions and two polycations in aqueous solution to compare with proposed complex formation mechanism.