Multifunctional two-photon excitable fluorescence probes for monitoring mitochondrial dynamics

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Several fluorescence probes for imaging of live mitochondria have been synthesized. These probes exhibited not only salient two-photon excitation fluorescence efficiencies but also high solubility to water. Owing to the high solubility to water of the novel probes, living cells have been stained without using any organic solvent. This advantage of the present probes enabled long time live cell imaging over than one week with a prominent sensitivity.In addition, we have developed fluorescence probes which switch accumulated organelle by external stimuli. The probes accumulated at mitochondria when the mitochondrial membrane potential was high. As the cell activity decreased the probes moved from mitochondria to nucleus. Reversible change of accumulated organelle has also been confirmed.