Multifunctional two-photon excitable fluorescence probes for monitoring mitochondrial dynamics

已经合成了几种用于活线粒体成像的荧光探针。这些探针不仅表现出突出的双光子激发荧光效率，而且对水的高溶解度。由于新型探针对水的高溶解度，活细胞已在不使用任何有机溶剂的情况下染色。本发明的探针的这一优点使得能够在超过一周的时间内以突出的灵敏度进行长时间活细胞成像。此外，我们已经开发了通过外部刺激来切换积累的细胞器的荧光探针。当线粒体膜电位高时，探针在线粒体处积累。随着细胞活性降低，探针从线粒体移动到细胞核。积累的细胞器的可逆变化也得到了证实。

Several fluorescence probes for imaging of live mitochondria have been synthesized. These probes exhibited not only salient two-photon excitation fluorescence efficiencies but also high solubility to water. Owing to the high solubility to water of the novel probes, living cells have been stained without using any organic solvent. This advantage of the present probes enabled long time live cell imaging over than one week with a prominent sensitivity.In addition, we have developed fluorescence probes which switch accumulated organelle by external stimuli. The probes accumulated at mitochondria when the mitochondrial membrane potential was high. As the cell activity decreased the probes moved from mitochondria to nucleus. Reversible change of accumulated organelle has also been confirmed.