Analysis of auxin-induced activation of the plasma membrane H+-ATPase

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In this study, we showed that auxin induces phosphorylation of the penultimate Thr in the plasma membrane H+-ATPase without involvement of the identified auxin receptors, TIR1/AFB, in Arabidopsis etiolated seedlings, and that auxin-induced phosphorylation is required for the early phase of auxin-induced hypocotyl elongation. Now, we are trying to identify the unidentified auxin receptor by reverse genetic and biochemical approaches. In addition, we have started to screen the mutants, which show reduced auxin-induced hypocotyl elongation and phosphorylation of the plasma membrane H+-ATPase in EMS-treated Arabidopsis etiolated seedlings.