Transcription profiles of host plant genes induced in the rhizobial and arbuscular mycorrhizal symbioses of soybean root

本研究分析了根瘤菌、丛枝菌根和双接种诱导的寄主基因转录谱，以阐明参与大豆根系双共生建立的寄主基因。结果表明，在根瘤菌、菌根和双接种条件下，寄主植物共有34个基因被诱导，这些基因是建立双共生关系所必需的。实时荧光定量pcr检测1型金属硫蛋白和CPRD49基因的表达。确定了1型金属硫蛋白基因在大豆根瘤和菌根中被显著诱导表达。我们试图利用启动子-gus基因融合来检测1型金属硫蛋白基因在转基因毛状根中的空间表达模式。

In this study, the transcription profiles of host genes induced by rhizobial, arbuscular mycorrhizal and dual inoculations were analyzed to elucidate the host genes involving in the establishment of the dual symbiosis in soybean root. We found that 34 genes of host plant were induced commonly by rhizobial, mycorrhizal and dual inoculations, suggesting that these genes are essential for establishing dual symbiosis. The gene expression of type1 metallothionein and CPRD49 were examined using real-time RT-PCR. It was ascertained that the expression of type1 metallothionein gene was significantly induced in the root nodule and mycorrhizal root of soybean. We tried to examine the spatial expression patterns of type1 metallothionein gene in the transgenic hairy roots using promoter-gus gene fusions.