Transcription profiles of host plant genes induced in the rhizobial and arbuscular mycorrhizal symbioses of soybean root

本研究通过对根瘤菌、丛枝菌根和双接种诱导的寄主基因转录谱的分析，阐明了参与大豆根际二元共生关系建立的寄主基因。我们发现，根瘤菌、菌根和双接种共同诱导了寄主植物的34个基因，表明这些基因是建立双重共生所必需的。用实时荧光定量RT-PCR方法检测细胞中金属硫蛋白和CPRD49基因的表达。结果表明，1类金属硫蛋白基因在大豆根瘤和菌根中的表达受到显著诱导。我们试图利用启动子-GUS基因融合的方法检测第一型金属硫蛋白基因在转基因毛状根中的空间表达模式。

In this study, the transcription profiles of host genes induced by rhizobial, arbuscular mycorrhizal and dual inoculations were analyzed to elucidate the host genes involving in the establishment of the dual symbiosis in soybean root. We found that 34 genes of host plant were induced commonly by rhizobial, mycorrhizal and dual inoculations, suggesting that these genes are essential for establishing dual symbiosis. The gene expression of type1 metallothionein and CPRD49 were examined using real-time RT-PCR. It was ascertained that the expression of type1 metallothionein gene was significantly induced in the root nodule and mycorrhizal root of soybean. We tried to examine the spatial expression patterns of type1 metallothionein gene in the transgenic hairy roots using promoter-gus gene fusions.