Study on the function of ND1 subunit, a key subunit of respiratory complex I

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To elucidate the functional and structural aspects of the ND1 subunit, which is a key subunit of the membrane domain of bovine heart mitochondrial complex I (NADH-quinone oxidoreductase), I synthesized unique inhibitor probes acting to the ND1 and identified their binding sites via photoaffinity labeling experiment. In addition, I succeeded in site-specific chemical labeling (alkynylation) via ligand-directed tosyl (LDT) chemistry using acetogenin as a high affinity ligand against complex I.