Development of conductive quinone-modified Au-electrode enabling super-sensitive detection of the activities of mitochondrial respiratory enzymes

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Development of an assay system, which enables super-sensitive detection of the activities of respiratory enzymes in mitochondrial membrane, is very useful for the bioenergetic studies of highly limited amount of preparative mitochondria such as human tissues. To this end, we tried to make a conductive quinone-modified Au-electrode, which can detect the enzyme activities by monitoring a change in the redox-state of"quinone pool"of inner mitochondrial membrane.