Elucidation of Selenium Metabolism in mammalian cells by speciation

通过物种形成阐明哺乳动物细胞中的硒代谢

• 批准号: 23390032
• 负责人: OGRA Yasumitsu
• 金额: $ 10.82万
• 依托单位: Showa Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390032/
• 关键词: セレン; スペシエーション; セレノシアネート; ICP-MS; Q-TOF; ESI-MS; テルル; セレン糖; ESI-MS-MS; セレノシアン酸; HPLC; LC-MS; セレノプロテイン; メタロチオネイン

When human hepatoma HepG2 cells were exposed to sodium selenite, an unknown selenium metabolite was detected in the cytosolic fraction by HPLC coupled with an inductively coupled plasma mass spectrometer (ICP-MS). The unknown selenium metabolite was also detected in the mixture of HepG2 homogenate and sodium selenite in the presence of exogenous glutathione (GSH). The unknown selenium metabolite was identified as selenocyanate by electrospray ionization mass spectrometry (ESI-MS) and ESI quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS). Selenocyanate was less toxic to HepG2 cells than selenite or cyanide, suggesting that it was formed to reduce the toxicity of selenite. On the other hand, selenocyanate could be assimilated to selenoproteins and selenometabolites in rats. Consequently, selenite was metabolized to selenocyanate to temporarily ameliorate its toxicity, and selenocyanate acted as an intrinsic selenium pool.

当人肝癌HepG 2细胞暴露于亚硒酸钠，未知的硒代谢产物中检测到的胞质组分的HPLC耦合电感耦合等离子体质谱仪（ICP-MS）。在外源性谷胱甘肽（GSH）存在下，在HepG 2匀浆和亚硒酸钠的混合物中也检测到未知的硒代谢产物。通过电喷雾质谱（ESI-MS）和ESI四极杆飞行时间质谱（ESI-Q-TOF-MS）鉴定未知硒代谢物为硒氰酸盐。硒氰酸盐对HepG 2细胞的毒性低于亚硒酸盐或氰化物，表明其形成是为了降低亚硒酸盐的毒性。另一方面，硒氰酸盐在大鼠体内可被同化为硒蛋白和硒代蛋氨酸。因此，亚硒酸盐被代谢为硒氰酸盐，暂时减轻其毒性，硒氰酸盐作为一个内在的硒库。

项目成果

Research tools and techniques for copper metabolism in mammals

哺乳动物铜代谢的研究工具和技术

• 发表时间: 2011
• 期刊: J. Health Sci
• 作者: 津川尚子;田邉はるか;安福良太;岡野登志夫;Y. Ogra
• 通讯作者: Y. Ogra

Speciation of selenium metabolites in Brassica family plants exposed with organoselenium compounds

有机硒化合物暴露下芸苔科植物中硒代谢物的形态

• 发表时间: 2011
• 作者: Ayako Yawata;Ayane Katayama;Marina Hayashi;Yasumi Anan;Yasumitsu Ogra
• 通讯作者: Yasumitsu Ogra

Speciation of selenometabolites in cultured cells exposed with selenite

暴露于亚硒酸盐的培养细胞中硒代代谢物的形态

• 发表时间: 2011
• 作者: Ren Koike;Marina Hayashi;Ayako Yawata;Yasumi Anan;Yasumitsu Ogra
• 通讯作者: Yasumitsu Ogra

主宰研究室

领先的研究实验室

ラットにおける新規セレン代謝物セレノシアネートの生体内挙動の解析

新型硒代谢物硒氰酸盐在大鼠体内的行为分析

• 发表时间: 2013
• 作者: 長谷川早喜;德本真紀;阿南弥寿美;小椋康光
• 通讯作者: 小椋康光