Basic analysis of pathogenesis of whooping cough

为了解百日咳、百日咳杆菌感染后出现阵发性咳嗽的机制，本研究采用B法复制了百日咳、百日咳杆菌感染后出现阵发性咳嗽的动物模型。支气管败血症，这是密切相关的B。百日咳。 该模型动物在鼻内感染500 cfu B后出现咳嗽。支气管炎在对B.在支气管败血症感染中，我们成功地分离了在模型动物中不诱导咳嗽的突变菌株。突变菌株和亲本野生型菌株的基因组比较显示在命名为crg1的基因中有一个单碱基插入。发现crg 1缺陷株不引起动物的阵发性咳嗽，表明crg 1可能参与了阵发性咳嗽。进一步的工作正在进行中，以确定crg1基因的特征。

To understand the mechanism of paroxysmal cough observed in whooping cough, Bordetella pertussis infection, we established an animal model that reproduce the paroxysmal cough by using B. bronchiseptica, which is closely related to B. pertussis. This model animal showed coughing after intranasal infection by 500 cfu of B. bronchiseptica. In the course of analyzing the B. bronchiseptica infection, we succeeded in isolating a mutant strain that did not induce coughing in the model animal. A comparison of the genomes of the mutant strain and the parental wild type strain revealed a single-base insertion in a gene designated crg1. A strain deficient in crg1 was found not to cause the paroxysmal cough in the animals, showing that crg1 might be involved in the paroxysmal cough. Further work is in progress to characterize the crg1 gene.