Development of a novel technique to monitor gene expression profiles of pathogenic bacteria during infection process

点击翻译按钮获取中文摘要

Analyses for gene expression of pathogens in response to the host environment provide clues to understanding the underlying events to establish the infectious diseases. In this study, we established a novel method named IVET-IP (in vivo expression tag immunoprecipitation system) to enable us to identify genes expressed in the host environment. By using this method, we determined genes expressed in Bordetella bronchiseptica in rats during infection process. As a result, we found that the in vivo expression pattern of bacterial virulence genes were quite different from that in vitro. In addition, at least 289 gene loci of Bordetella bronchiseptica were found to be expressed >2-fold in rats compared to in vitro.