Establishment of therapeutic lymphangiogenesis for lymphedema

淋巴水肿是癌症手术切除后发生的严重临床问题。本文报道了从C57 BL/6 J小鼠中分离的脂肪再生细胞（ADRCs）移植到小鼠水肿模型中可诱导淋巴管生成。为了检查ADRC植入体内的功效，我们建立了一种新的小鼠尾部水肿模型。局部注射ADRCs可明显改善淋巴水肿。组织学分析显示，ADRC组的毛细淋巴管密度大于PBS注射对照组。ADRC组血管内皮生长因子C（VEGF-C）的组织表达高于对照组。ADRC释放VEGF-C，刺激淋巴管生成。ADRC的植入也增强了M2巨噬细胞的募集，M2巨噬细胞充当淋巴管内皮祖细胞。 总之，ADRCs的植入可能是一个有用的治疗方案，通过调解淋巴管生成水肿。

Lymphedema is a serious clinical problem that occurs after surgical resection of cancers. We report that implantation of adipose-derived regenerative cells (ADRCs) can induce lymphangiogenesis in a mouse model of lymphedema.ADRCs were isolated from C57BL/6J mice. To examine the efficacy of ADRC implantation in vivo, we established a new mouse model of tail lymphedema. Lymphedema was improved significantly by local injection of ADRCs. Histological analysis revealed that lymphatic capillary density was greater in the ADRC group than in the PBS-injected control group. Tissue expression of vascular endothelial growth factor C (VEGF-C) was greater in the ADRC group than in the control group. ADRCs released VEGF-C, which stimulated lymphangiogenesis. Implantation of ADRCs also enhanced recruitment of M2 macrophages, which served as lymphatic endothelial progenitors. In conclusion, implantation of ADRCs could be a useful treatment option for lymphedema by mediation of lymphangiogenesis.