Study on the reactivation-induced development of AIDS-related cryptococcosis using a mouse model

新型隐球菌是一种重要的条件致病真菌，可引起艾滋病患者严重的脑膜脑炎。最近，人们认为隐球菌病是在免疫功能低下的情况下，通过持续感染的病原体的再激活而发展的。在这项研究中，我们研究了记忆T（Tm）细胞的动力学在感染的肺部使用小鼠模型与持续感染的C。新人类此外，我们成功地建立了一种新的转基因小鼠表达T细胞受体，识别MP 98，这种真菌病原体的主要T细胞抗原。在这种小鼠中，IFN-γ的合成和C.感染后，新生儿在肺部的速度明显加快。这使得有可能更准确地检查Tm细胞的动力学以及在持续感染的C.新人类

Cryptococcus neoformans is an important opportunistic fungal pathogen, which causes severe meningoencephalitis in AIDS patients. Recently, it has been thought that cryptococcosis develops by reactivation of persistently infected pathogen under an immunocompromised condition. In this study, we examined the kinetics of memory T (Tm) cells in the infected lungs using a mouse model with persistent infection with C. neoformans. In addition, we succeeded in establishing a novel transgenic mouse expressing T cell receptor which recognizes MP98, a major T cell antigen of this fungal pathogen. In this mouse, both IFN-gamma synthesis and clearance of C. neoformans were strikingly accelerated in the lungs after infection. This makes it possible to more accurately examine the kinetics of Tm cells and the effect on the Tm cell response during reactivation of persistently infected C. neoformans.