The elucidation of the early immune system in percutaneous infection of HSV and HIV and the development of novel methods to prevent their cutaneous infection

阐明 HSV 和 HIV 经皮感染的早期免疫系统并开发预防其皮肤感染的新方法

• 批准号: 23390282
• 负责人: SHIMADA Shinji
• 金额: $ 12.48万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390282/
• 关键词: HSV; HIV; 経皮感染; ランゲルハンス細胞; 抗菌ペプチド; 性行為感染症; 自然免疫

HSV-2 shedding is associated with increased risk for sexually acquiring HIV. Because Langerhans cells (LCs) are suspected to be the initial target cells infected by HIV following sexual exposure, we studied whether HSV-2 affects HIV infection of LCs. HSV-2 enhanced HIV susceptibility in LCs within skin explants, even though relatively few HSV-2/HIV co-infected LCs were detected, suggesting that HSV-2 enhances HIV infection of LCs through indirect mechanisms. Indeed, HSV-2 stimulated production of antimicrobial peptides (AMPs) by epithelial cells, including human defensins and LL-37, and the latter strongly enhanced HIV susceptibility in LCs by up-regulating HIV receptors. Culture supernatants of epithelial cells infected with HSV-2 enhanced HIV susceptibility in LCs, and this effect was abrogated by blocking LL-37 production. These data suggest that HSV-2 enhances sexual transmission of HIV by increasing HIV susceptibility of LCs via epithelial cell production of LL-37.

HSV-2脱落与通过性行为获得HIV的风险增加有关。 由于郎格罕细胞（LC）被怀疑是HIV感染后的最初靶细胞，我们研究了HSV-2是否影响LC的HIV感染。 HSV-2增强了皮肤外植体内LC中的HIV易感性，即使检测到相对较少的HSV-2/HIV共感染LC，表明HSV-2通过间接机制增强LC的HIV感染。 事实上，HSV-2刺激上皮细胞产生抗菌肽（AMP），包括人防御素和LL-37，后者通过上调HIV受体强烈增强LC中的HIV易感性。 HSV-2感染的上皮细胞培养上清增强了LC对HIV的易感性，而阻断LL-37的产生可消除这种效应。 这些数据表明HSV-2通过上皮细胞产生LL-37增加LC的HIV易感性来增强HIV的性传播。

项目成果

Gutter method : noninvasive management of ingrown nails caused by epidermal growth factor inhibitor treatment

Gutter法：表皮生长因子抑制剂治疗引起的指甲内嵌的无创治疗

• 发表时间: 2012
• 期刊: Clin Exp Dermatol
• 影响因子: 4.1
• 作者: Harada K;Yamaguchi M;Miyajima S;Kawamura T;Shibagaki S;Shimada S
• 通讯作者: Shimada S

Antimicrobial Peptide LL-37 Produced by HSV-2-Infected Keratinocytes Enhances HIV Infection of

HSV-2 感染的角质形成细胞产生的抗菌肽 LL-37 可增强 HIV 感染

• 发表时间: 2013
• 期刊: Cell Host & Microbe
• 影响因子: 30.3
• 作者: 小川陽一;川村龍吉;松澤高光;青木類;Peter Gee; 山下篤哉; 森石恆司;山崎研志;小柳義夫;Andrew Blauvelt;島田眞路
• 通讯作者: 島田眞路

皮膚科学講座 - 山梨大学 医学部

皮肤科课程 - 山梨大学医学部

Two cases of autosomal recessive woolly hair with LIPH gene mutations

常染色体隐性羊毛状毛发LIPH基因突变2例

• DOI: 10.1111/j.1365-4632.2012.05775.x
• 发表时间: 2013
• 期刊: Int J Dermatol
• 影响因子: 3.6
• 作者: Harada K;Inozume T;Kawamura T;Shibagaki N;Kinoshita T;Deguchi N;Shimada S
• 通讯作者: Shimada S

A case of oral mucosal fixed eruption caused by methacrylate

甲基丙烯酸酯致口腔粘膜固定性出疹1例

• DOI: 10.1111/cod.12183
• 发表时间: 2014
• 期刊: Contact Dermatitis
• 影响因子: 5.5
• 作者: Inozume T;Nakazawa R;Tanaka K;Harada K;Kawamura T;Shibagaki N;Shimada S
• 通讯作者: Shimada S