Control of liver fibrosis after transplantation by immunomodulation

通过免疫调节控制移植后肝纤维化

• 批准号: 23390322
• 负责人: KOJIRO Taura
• 金额: $ 12.23万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390322/
• 关键词: 肝線維化; 肝移植; 脾臓; 免疫; 肝線進化; 星細胞; Helper T細胞; サイトカイン

To investigate the effects of splenectomy on liver fibrosis, BALB/c mice underwent splenectomy or sham operation and subjected to carbon tetrachloride (CCl4) or thioacetamide (TAA) treatments. Splenectomy significantly suppressed liver fibrosis in the CCl4 and TAA models. The inhibitory effect of splenectomy on liver fibrosis was not observed in SCID mice.To investigate the mechanisms underlying these effects, we investigated the change of liver Th1/Th2 balance. As a result, splenectomy shifted Th1/Th2 balance toward Th1-dominance. In addition, the number of CD4+ lymphocytes in the spleen was decreased. In contrast, that in the liver was increased. GFP+ splenic lymphocytes were transplanted into the spleen of wild type mice. GFP+CD4+ lymphocytes appeared in the liver after induction of fibrosis and the vast majority of them were Th2 lymphocytes by flow cytometry.We conclude that splenectomy suppresses progression of liver fibrosis at least partly through restoring the Th1/Th2 balance.

为了研究脾切除术对肝纤维化的影响，BALB/C小鼠进行了脾切除术或假手术，并接受四氯化碳（CCL4）或硫乙酰氨酰胺（TAA）处理。脾切除术显着抑制了CCL4和TAA模型中的肝纤维化。在SCID小鼠中未观察到脾切除术对肝纤维化的抑制作用。为了研究这些作用的机制，我们研究了肝TH1/TH2平衡的变化。结果，脾切除术将Th1/Th2的平衡转移到Th1占主导地位。另外，脾脏中CD4+淋巴细胞的数量减少。相比之下，肝脏中的肝脏增加了。将GFP+脾淋巴细胞移植到野生型小鼠的脾脏中。 GFP+ CD4+淋巴细胞在诱导纤维化后出现在肝脏中，绝大多数是通过流式细胞仪Th2淋巴细胞。我们的结论是，脾切除术至少通过恢复TH1/TH2平衡来抑制至少部分部分地抑制肝纤维化的进展。

项目成果

Plissimo Eraを用いた 肝切除術のVirtual Reality

使用 Plissimo Era 进行肝脏切除的虚拟现实

• 发表时间: 2012
• 作者: Song J. Hagiya H.;Kurata H.;Mizuno H.;Ito T.;宇野 太;田浦 康二朗
• 通讯作者: 田浦 康二朗

Origin of myofibroblasts in liver fibrosis.

• DOI: 10.1186/1755-1536-5-s1-s17
• 发表时间: 2012
• 期刊: Fibrogenesis & tissue repair
• 作者: Brenner DA;Kisseleva T;Scholten D;Paik YH;Iwaisako K;Inokuchi S;Schnabl B;Seki E;De Minicis S;Oesterreicher C;Taura K
• 通讯作者: Taura K

Usefulness of Liver Stiffness Measurement for the Prediction of Posthepatectomy Liver Failure

肝脏硬度测量对于预测肝切除术后肝衰竭的有用性

• 发表时间: 2012
• 作者: Noda T. Yamamoto H.;Takemasa I.;Yamada D.;Uemura M.;Wada H.;Kobayashi S.;Marubashi S.;Eguchi H.;Tanemura M.;Umeshita K.;Doki Y.;Mori M.;Nagano H;Taura Kojiro
• 通讯作者: Taura Kojiro

Liver Stiffness Measurement Predicts Posthepatectomy Liver Failure

肝脏硬度测量可预测肝切除术后肝衰竭

• 发表时间: 2014
• 作者: K. Taura;Y. Koyama;K. Tanabe;G. Yamamoto;T. Nishio;E. Hatano;S. Uemoto
• 通讯作者: S. Uemoto

Three-Dimensional Image of Hepatic Hilum by CT Cholangiography with Carbon Dioxide for Preoperative Assessment of Biliary Malignancies

二氧化碳 CT 胆道造影的肝门三维图像用于胆道恶性肿瘤的术前评估

• 发表时间: 2012
• 作者: Masuzawa T.Fujiwara Y.;Okada K.;Nakamura A.;Takiguchi S.;Nakajima K.,Miyata H.;Yamasaki M.;Kurokawa Y.;Osawa R.;Takeda K.;Yoshida K.;Tsunoda T.;Nakamura Y.;Mori M.;Doki Y.;田浦 康二朗
• 通讯作者: 田浦 康二朗