Optimization of Regeneration Technique for Large Bone Defect

大骨缺损再生技术的优化

• 批准号: 23390364
• 负责人: MYOUI Akira
• 金额: $ 11.9万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390364/
• 关键词: 骨再生医療; 間葉系幹細胞; 化学療法; 悪性骨腫瘍; 骨芽細胞分化; 無血清培地; iPS細胞

We successfully purified LNRT cells and MUSE cells from the bone marrow mononuclear cells by the cell surface marker. However, in vitro amplification of those specific stem cells that was required for clinical application seemed difficult compared with classic mesenchymal stem cells (MSCs).After administration of doxorubicin, rat MSCs derived from bone marrow, fat, and muscle tissue were investigated for capacity of proliferation and differentiation. The negative impact of chemotherapy was least in adipose-derived MSCs.We succeeded in the art that a combination of factors such as seeding density of cells, surface treatment of the culture dish, and hypoxic culture condition differentiate mesenchymal progenitor cells from iPS cells safely and with high efficiency. In addition, we found that the cytokine gradient method in serum-free medium induced the progenitors into osteoblastic lineage.

我们通过细胞表面标记成功地从骨髓单个核细胞中纯化了LNRT细胞和MUSE细胞。然而，与经典的间充质干细胞（mesenchymal stem cells，MSCs）相比，这些特异性的干细胞在体外的扩增是非常困难的。在脂肪来源的MSC中，化疗的负面影响最小。我们在本领域中成功地将细胞的接种密度、培养皿的表面处理和低氧培养条件等因素组合，安全且高效地从iPS细胞分化间充质祖细胞。此外，我们发现无血清培养液中细胞因子梯度法可诱导祖细胞向成骨细胞方向分化。

项目成果

P38 mitogen-activated protein kinase inhibitor, FR167653, inhibits parathyroid hormone related protein-induced osteoclastogenesis and bone resorption.

• DOI: 10.1371/journal.pone.0023199
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Tao H;Okamoto M;Nishikawa M;Yoshikawa H;Myoui A
• 通讯作者: Myoui A

A novel angiomatoid epithelioid sarcoma cell line, Asra-EPS, forming tumors with large cysts containing hemorrhagic fluid in vivo.

• DOI: 10.1186/1756-0500-6-305
• 发表时间: 2013-08-01
• 期刊: BMC research notes
• 影响因子: 1.8
• 作者: Imura Y;Naka N;Outani H;Yasui H;Takenaka S;Hamada K;Ozaki R;Kaya M;Yoshida K;Morii E;Myoui A;Yoshikawa H
• 通讯作者: Yoshikawa H

BMPを用いた人工骨の高機能化

使用 BMP 改善人造骨的功能

• 作者: 大森信介;村瀬 剛;片岡利行;川西洋平;三宅 潤一;森友 寿夫;菅本一臣;吉川 秀樹;名井 陽;Hirata M;土 屋弘行;Muraki S;名井 陽
• 通讯作者: 名井 陽

人工骨の基礎と臨床

人造骨的基础和临床方面

• 发表时间: 2013
• 作者: 小林寛、川口浩;他;名井 陽
• 通讯作者: 名井 陽

Feasibility and limitations of the round robin test for assessment of in vitro chondrogenesis evaluation protocol in a tissue-engineered medical product

组织工程医疗产品体外软骨形成评估方案循环试验的可行性和局限性

• DOI: 10.1002/term.460
• 发表时间: 2011
• 期刊: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
• 影响因子: 3.3
• 作者: Masako Yokoi;et al.
• 通讯作者: et al.