Optimization of Regeneration Technique for Large Bone Defect

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We successfully purified LNRT cells and MUSE cells from the bone marrow mononuclear cells by the cell surface marker. However, in vitro amplification of those specific stem cells that was required for clinical application seemed difficult compared with classic mesenchymal stem cells (MSCs).After administration of doxorubicin, rat MSCs derived from bone marrow, fat, and muscle tissue were investigated for capacity of proliferation and differentiation. The negative impact of chemotherapy was least in adipose-derived MSCs.We succeeded in the art that a combination of factors such as seeding density of cells, surface treatment of the culture dish, and hypoxic culture condition differentiate mesenchymal progenitor cells from iPS cells safely and with high efficiency. In addition, we found that the cytokine gradient method in serum-free medium induced the progenitors into osteoblastic lineage.