Research and development of innovative caries treatment by biotooth transplantation using iPS cells

本研究的目的是诱导多能干细胞向成牙本质细胞分化的反应的三维硅膜支架与牙本质小管样孔的机械压缩。采用优化的方法和材料对膜进行预涂，以增强iPS细胞的附着。结果表明，在压力为19.6kPa、细胞密度为4.0 × 105 cells/cm 2、加载时间为9 h的条件下，成牙本质细胞分化率明显提高，成牙本质细胞特异性标志物的表达明显增加，细胞突起向膜孔内延伸的能力增强，这是成牙本质细胞的典型形态特征。此外，观察到BMP 7和Wnt 10a的上调以及MAPK信号通路的参与。这些结果表明，三维物理结构的支架在成牙本质细胞分化的重要性。

The present study aimed at the induction of iPS cells into odontoblastic differentiation in response to mechanical compression of the three-dimensional silicon membrane scaffold with dentinal tubule-like pores. The membrane was precoated with optimal method and materials to enhance iPS cell attachment. The optimal conditions, which were 19.6 kPa in compression magnitude, 4.0x10 5 cells/cm2 in cell density, and 9 hours of loading time, efficiently induced odontoblastic differentiation, showing significantly increased expression of odontoblast specific markers and enhanced elongation of cellular processes into the pore of membrane, a typical morphological feature of odontoblasts. In addition, up-regulation of BMP7 and Wnt10a and the involvement of the MAPK signaling pathway were observed. These results suggested the importance of three-dimensional physical structure of the scaffold in odontoblastic differentiation.