Recovery from spinal cord injury in the chronic phase -the mechanism of denosomin, a novel compound with multiactivities-

一种新的化合物，Denosomin，改善后肢运动功能障碍和轴突生长，特别是在5-羟色胺（5-HT）-阳性束穿过疤痕，并增加星形胶质细胞的密度。在培养的星形胶质细胞中，Denosomin增加星形胶质细胞增殖，抑制星形胶质细胞死亡，并增加波形蛋白的表达和分泌。此外，波形蛋白治疗增加轴突生长在培养的神经元，即使在抑制CSPG的存在下。在给予Denosomin的SCI小鼠的胶质瘢痕内，表达波形蛋白的星形胶质细胞增加，并且5-HT阳性轴突生长以波形蛋白相关的方式发生。我们首次发现波形蛋白直接刺激神经元上的IGF 1受体，并通过信号传导诱导轴突生长。

A novel compound, Denosomin, improved hindlimb motor dysfunction and axonal growth, especially in the 5-hydroxytryptamine (5-HT)-positive tracts across the scar and increased the density of astrocytes. Denosomin increased astrocyte proliferation, inhibited astrocytic death and increased the expression and secretion of vimentin in cultured astrocytes. Furthermore, vimentin treatment increased axonal outgrowth in cultured neurons, even in the presence of inhibitory CSPG. Vimentin-expressing astrocytes increased inside of the glial scars of SCI mice administered Denosomin, and 5-HT-positive axonal growth occurred in a vimentin-associated manner. We firstly found that vimentin stimulated directly IGF1 receptors on neurons and the signaling elicited the axonal growth.