Improvement of microinsemination techniques with primary spermatocytes of mice based on live-cell imaging analysis

基于活细胞成像分析的小鼠原代精母细胞显微授精技术的改进

基本信息

项目摘要

Microinsemination is a technique to deliver male germ cells directly into oocytes, and is a very effective technique to fertilize oocytes consistently, even male germ cells that are unable to fertilize oocytes under conventional in vovo and in vitro conditions. Although offspring can be obtained by primary spermatocyte injection in mice, its efficiency is still low (2-4%) compared with that by spermatid (30%) or by spermatozoa (40-50%). To know whether the poor full-term development, primary spermatocyte injected oocytes were monitored by live-cell imaging techniques. The gap of the chromosome division timing was observed between male and female chromosomes. And a histone, which are globally deacetylated in mammalian oocytes during meiosis, was remained acetylated in primary spermatocyte injected oocytes. Thus, inadequate histone deacetylation may be caused chromosome abnormalities and low oocyte developments.
显微授精是一种将雄性生殖细胞直接输送到卵母细胞中的技术,并且是一种非常有效的使卵母细胞持续受精的技术,即使是在常规的体内和体外条件下不能使卵母细胞受精的雄性生殖细胞。虽然小鼠初级精母细胞注射可以获得子代,但其效率(2-4%)与精子细胞(30%)或精子(40-50%)相比仍然较低。为了解初级精母细胞注射卵母细胞后是否发育不良,采用活细胞成像技术进行监测。在雌雄染色体之间观察到染色体分裂时间的差距。在哺乳动物卵母细胞减数分裂过程中,一种组蛋白被完全脱乙酰化,而在注射初级精母细胞的卵母细胞中,组蛋白仍保持乙酰化状态。因此,组蛋白去乙酰化不足可能导致染色体异常和卵母细胞发育迟缓。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Development of Microinsemination Techniques for Laboratory Animals and Its Practical Applications
  • DOI:
    10.1274/jmor.30.159
  • 发表时间:
    2013-11
  • 期刊:
  • 影响因子:
    0
  • 作者:
    N. Ogonuki
  • 通讯作者:
    N. Ogonuki
In vitro production of fertile sperm from murine spermatogonial stem cell lines
  • DOI:
    10.1038/ncomms1478
  • 发表时间:
    2011-09-01
  • 期刊:
  • 影响因子:
    16.6
  • 作者:
    Sato, Takuya;Katagiri, Kumiko;Ogawa, Takehiko
  • 通讯作者:
    Ogawa, Takehiko
Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect
人為的活性化処理なしでの凍結円形精子細胞の顕微注入によるマウス産仔の作出
显微注射冷冻圆形精子细胞无需人工活化处理产生小鼠幼崽
  • DOI:
  • 发表时间:
    2011
  • 期刊:
  • 影响因子:
    0
  • 作者:
    越後貫成美;ほか
  • 通讯作者:
    ほか
遺伝工学基盤後術室
基因工程术后室
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    0
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OGONUKI Narumi其他文献

OGONUKI Narumi的其他文献

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{{ truncateString('OGONUKI Narumi', 18)}}的其他基金

A high-speed congenic strategy using first-wave male germ cells
使用第一波雄性生殖细胞的高速同类策略
  • 批准号:
    20500383
  • 财政年份:
    2008
  • 资助金额:
    $ 3.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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