A high-speed congenic strategy using first-wave male germ cells

使用第一波雄性生殖细胞的高速同类策略

• 批准号: 20500383
• 负责人: OGONUKI Narumi
• 金额: $ 2.91万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20500383/
• 关键词: マウス; 実験動物; 発生工学; 顕微授精

To establish high-speed congenic system, microinsemination with round spermatid which retrieved from immature mouse selected by marker-assisted screening system was tried. Nine congenic strains were produced by this method for 106 and 190 days of N3 to N5 generation. The generation turnover time in mice can be shorted to about 40 days by using the first wave round spermatids as male gametes. This method can establish congenic strain the period below about half of conventional method.

为了建立快速的同种异体授精系统，尝试用标记辅助筛选系统筛选出的未成熟小鼠圆形精子细胞进行显微授精。用该方法从N3代到N5代分别进行了106和190天的传代，共获得9个同源菌株。用第一波圆形精子细胞作雄配子，可使小鼠的世代更替时间缩短到40天左右。该方法建立同源株的周期比常规方法缩短一半左右。

项目成果

Factors affecting the outcome of mouse ICSI : Factorial analysis by genotypes stage of male germ cells and their freezing treatment

影响小鼠 ICSI 结果的因素：雄性生殖细胞基因型阶段及其冷冻处理的因子分析

• 发表时间: 2010
• 作者: Ogonuki N;et.al.
• 通讯作者: et.al.

顕微授精技術を利用したマウス超迅速コンジェニック化技術の確立

利用显微授精技术建立超快速小鼠同类技术

• 发表时间: 2009
• 作者: 越後貫成美;ほか
• 通讯作者: ほか

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Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes.

显微注射到兔卵母细胞中的精子和精子细胞中心体的功能评估。

• 发表时间: 2009
• 期刊: Mol Reprod Dev 76
• 作者: Tachibana M;Terada Y;Ogonuki N;Ugajin T;Ogura A;Murakami T;Yaegashi N;Okamura K.
• 通讯作者: Okamura K.

Ultrastructure of placental hyperplasia in mice : Comparison of placental phenotypes with three different etiologies

小鼠胎盘增生的超微结构：三种不同病因的胎盘表型比较

• 发表时间: 2008
• 期刊: Placenta 29(8)
• 作者: Wakisaka;N.;Ogonuki;N.;Miki;Y.;Sekita;Y.;Hanaki;K.;Aikatsuka;A.;Kaneko-Ishino;T.;Ishino F.;Ogura;A.
• 通讯作者: A.