Effect of passive smoking on chromosome aberration and DNA damage in alveolar macrophage

被动吸烟对健康的影响是一个重要的社会问题。在这项研究中，我们利用被动吸烟（香烟侧流烟雾暴露自动机）设备研究了被动吸烟对肺泡巨噬细胞染色体畸变和DNA损伤的影响。吸烟后小鼠肺泡巨噬细胞恢复正常。CGH法检测肺泡巨噬细胞的染色体畸变和DNA损伤。染色体畸变显著增强的部分有116处，染色体畸变不足的部分有169处。基于这些数据，我们提取了10个显著畸变部分的基因组。显著扩增的基因组片段为chr8的qE1、chrX的qF2、chr6的qG1、chr17的qA3。相反，显著缺陷部分为chr3的qE3、chr5的qC3.1、chr13的qB3和chr16的qB3。

The influence on health by the passive smoking is an important social problem. In this study, we investigated the effect of passive smoking on the chromosome aberration and DNA damage of alveolar macrophage using passive smoking (cigarette side-stream smoke exposure automatic machine) facility. After smoking, alveolar macrophage was recovered from mice. Chromosome aberration and DNA damage of alveolar macrophages were examined by CGH assay. The significant enhancements of chromosome aberrations were 116 parts, and the deficiencies of chromosome aberrations were 169 parts. Based on these data, we extracted the genome of 10 parts of significantly aberrations. The significantly amplified genome parts were qE1 of chr8, qF2 of chrX, qG1 of chr6, qA3 of chr17. On the contrary, the significantly defective genome parts were qE3 of chr3, qC3.1 of chr5, qB3 of chr13 and qB3 of chr16.