Effect of passive smoking on chromosome aberration and DNA damage in alveolar macrophage

被动吸烟对肺泡巨噬细胞染色体畸变和DNA损伤的影响

• 批准号: 23500826
• 负责人: TAKEUCHI Minoru
• 金额: $ 3.33万
• 依托单位: Kyoto Sangyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23500826/
• 关键词: 受動喫煙; 副流煙; 肺胞マクロファージ; 染色体異常; 遺伝子損傷; CGHマイクロアレイ; 活性酸素; 副流煙粒子

The influence on health by the passive smoking is an important social problem. In this study, we investigated the effect of passive smoking on the chromosome aberration and DNA damage of alveolar macrophage using passive smoking (cigarette side-stream smoke exposure automatic machine) facility. After smoking, alveolar macrophage was recovered from mice. Chromosome aberration and DNA damage of alveolar macrophages were examined by CGH assay. The significant enhancements of chromosome aberrations were 116 parts, and the deficiencies of chromosome aberrations were 169 parts. Based on these data, we extracted the genome of 10 parts of significantly aberrations. The significantly amplified genome parts were qE1 of chr8, qF2 of chrX, qG1 of chr6, qA3 of chr17. On the contrary, the significantly defective genome parts were qE3 of chr3, qC3.1 of chr5, qB3 of chr13 and qB3 of chr16.

被动吸烟对健康的影响是一个重要的社会问题。本研究利用被动吸烟（香烟侧流烟雾暴露自动机）装置，观察了被动吸烟对肺泡巨噬细胞染色体畸变和DNA损伤的影响。吸烟后，从小鼠中回收肺泡巨噬细胞。CGH法检测肺泡巨噬细胞染色体畸变和DNA损伤。染色体畸变显著增强的有116个，染色体畸变缺失的有169个。根据这些数据，我们提取了10个显著畸变部分的基因组。显著扩增的基因组片段为chr8的qE1、chrX的qF2、chr6的qG1、chr17的qA3。相反，显著缺陷的基因组部分是chr3的qE3、chr5的qC3.1、chr13的qB3和chr16的qB3。

项目成果

Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils.

姬松茸热水提取物对中性粒细胞趋化性的影响。

• DOI: 10.4236/jcdsa.2013.31a003
• 发表时间: 2013
• 期刊: JCDSA
• 作者: 重吉瑛里;竹内実;Hirono Y.;Sakura M.;Miyagawa M.
• 通讯作者: Miyagawa M.

Cigarette smoke induce alteration of structure and function in alveolar macrophages

香烟烟雾引起肺泡巨噬细胞结构和功能的改变

• 发表时间: 2013
• 期刊: IJBBB
• 作者: Hirono Y;Kawazoe A;Nose M;Sakura M;Takeuchi M.
• 通讯作者: Takeuchi M.

Environmental mutagens may be implicated in emergence of drug-resistant microorganisms.

环境诱变剂可能与耐药微生物的出现有关。

• 发表时间: 2011
• 期刊: FEMS Microbiology Letters
• 影响因子: 2.1
• 作者: Miyahara;Emiko; Nishie;Makiko; Takumi;Shota; Miyanohara,Hiroaki; Nishi;Junichiro; Yoshiie;Kiyotaka; Oda;Hiroshi; Takeuchi;Minoru et al.
• 通讯作者: Minoru et al.

Pinkerton. Cigarette Smoke as Environmental Factor Induce Inhibition of Immune Functions and DNA damage in Alveolar Macrophages

平克顿。

• 发表时间: 2013
• 作者: M. Takeuchi;Y. Hirono;M. Nose;S. Inoue;K. Sasaki;KE
• 通讯作者: KE

Effect Of Side-Stream Tobacco Smoke (STS) On Immune Functions And DNA Damage In Alveolar Macrophage (AM)

侧流烟草烟雾 (STS) 对肺泡巨噬细胞 (AM) 免疫功能和 DNA 损伤的影响

• 发表时间: 2012
• 作者: M. Takeuchi;S. Inoue;Y. Hirono;A. Kawazoe;E. Shigeyoshi;M. Nose;S. Inaga;K.E. Pinkerton
• 通讯作者: K.E. Pinkerton