Effect of smoking on DNA damage and chromosome aberration in alveolar macrophage

吸烟对肺泡巨噬细胞DNA损伤和染色体畸变的影响

• 批准号: 17500488
• 负责人: TAKEUCHI Minoru
• 金额: $ 2.4万
• 依托单位: Kyoto Sangyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17500488/
• 关键词: Smoking; Alveolar macrophage; DNA damage; Chromosome aberration; Genetic aberration; 遺伝子損傷; タバコ喫煙

We examined concerning the effect of the smoking on the gene (DNA) damage and the chromosome aberration of the alveolar macrophage from 02- production of the alveolar macrophage being strengthened by smoking. The DNA damage and chromosome aberration of the alveolar macrophage was caused by smoking. The chromosome aberration of was presented in all the chromosomes in alveolar macrophage. We examined about 43000 genes from unusual points of chromosomes, the number of total genes which aberration points were detected was 280 genes compared with non-smoking, and the number of genes which increased more than 2 times expression was 127, however, genes expressions which decreased more than 2 times were 153 compared with non-smoking. Specially, the gene expression increased by smoking was chemokine genes, conversely the gene expressions decreased by smoking were adhesion molecules and the prostacyclin synsetiyze enzyme. Moreover, increase in 8-OHdG which is closely associated with carcinogenesis was detected in DNA of lung tissue by smoking, the expression of 8-OHdG was recognized on mucous epithelial cells in bronchiole. These results suggested that the mechanism of inhibition of immune functions in alveolar macrophage by smoking was associated with caused DNA damage by generated OH through H_2O_2 from O_2^-, and its results was occurred inhibition of expression of antigen presenting molecule and inhibition of cytokine production. It was suggested that increase in the generation of 8-OHdG by excessively reactive oxygen species (ROS) from alveolar macrophage by smoking and inhibition of immune function were related to incidence of lung cancer. Furthermore, the repression of immune function of alveolar macrophage by cigarette smoke influenced the early stage of B cell, and its result inhibited antibody production, and these results may be present the possibility to increase for infection.

我们研究了吸烟对肺泡巨噬细胞基因(DNA)损伤和染色体畸变率的影响--吸烟增强了肺泡巨噬细胞的产生。吸烟可引起肺泡巨噬细胞DNA损伤和染色体畸变。肺泡巨噬细胞的所有染色体均出现染色体异常。我们从染色体的异常点检测了约43000个基因，与不吸烟相比，总共检测到了280个基因，其中表达增加2倍以上的基因有127个，而表达降低2倍以上的基因有153个。特别是，吸烟导致的基因表达增加是趋化因子基因，相反，吸烟降低的基因表达是黏附分子和前列环素合成酶。此外，吸烟可使肺组织DNA中与癌变密切相关的8-OHdG表达增加，8-OHdG表达于细支气管镜下粘膜上皮细胞。以上结果提示，吸烟抑制肺泡巨噬细胞免疫功能的机制与O2~-通过H_2O_2产生的OH引起DNA损伤有关，其结果是抑制了抗原提呈分子的表达，抑制了细胞因子的产生。提示吸烟引起的肺泡巨噬细胞产生的过量ROS增加了8-OHdG的生成和免疫功能的抑制与肺癌的发生有关。此外，香烟烟雾对肺泡巨噬细胞免疫功能的抑制影响了B细胞的早期阶段，其结果抑制了抗体的产生，这些结果可能为感染提供了增加的可能性。

项目成果

Generation of monochlorofluorescein from dichlorofluorescin by photo-irradiationdagger

光照射匕首从二氯荧光素生成一氯荧光素

• 发表时间: 2006
• 作者: Rollin P.;Takeuchi M.;竹内実;Bei Yu;Bei Yu;竹内 実;M.Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Takeuchi Minoru;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;M. Takeuchi;竹内 実;竹内 実;竹内 実;竹内 実;Minorcu Takeuchi;Minorcu Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi
• 通讯作者: Minoru Takeuchi

Effect of water extract from Agaricus blazei murill on induction of peritoneal cells and prevention of tumor growth

姬松茸水提取物诱导腹膜细胞和预防肿瘤生长的作用

• 发表时间: 2005
• 作者: Rollin P.;Takeuchi M.;竹内実;Bei Yu;Bei Yu;竹内 実;M.Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Takeuchi Minoru;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;M. Takeuchi;竹内 実;竹内 実;竹内 実;竹内 実;Minorcu Takeuchi;Minorcu Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;竹内 実;TAKEUCHI Minoru;Minoru Takeuchi;Minoru Takeuchi
• 通讯作者: Minoru Takeuchi

Interleukin‐21 triggers both cellular and humoral immune responses leading to therapeutic antitumor effects against head and neck squamous cell carcinoma

• DOI: 10.1002/jgm.817
• 发表时间: 2006-01
• 期刊: The Journal of Gene Medicine
• 作者: H. Nakano;T. Kishida;H. Asada;M. Shin-Ya;T. Shinomiya;J. Imanishi;Taketoshi Shimada;S. Nakai;M. Takeuchi;Y. Hisa;O. Mazda

Exposure to Cigarette Smoke Induces Oxidative DNA Damage and Suppresses Antigen-Presenting Activity in Alveolar Macrophages.

接触香烟烟雾会诱导氧化 DNA 损伤并抑制肺泡巨噬细胞中的抗原呈递活性。

• 发表时间: 2007
• 作者: Rollin P.;Takeuchi M.;竹内実;Bei Yu;Bei Yu;竹内 実;M.Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Minorcu Takeuchi;Takeuchi Minoru;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi;Minoru Takeuchi
• 通讯作者: Minoru Takeuchi

喫煙による抗体産生制御.

通过吸烟控制抗体的产生。

• 发表时间: 2007
• 期刊: 臨床免疫・アレルギー科 11月号 48
• 作者: Katshuhiro Sumi;et al.;竹内 実;Nagai S.;Nagai S;竹内実
• 通讯作者: 竹内実