Purification of a new PCE-dechlorinating enzyme and microbial community structure analysis under remediation of PCE-contaminated soil

新型四氯乙烯脱氯酶的纯化及四氯乙烯污染土壤修复下的微生物群落结构分析

• 批准号: 23510086
• 负责人: CHANG Young-Cheol
• 金额: $ 3.49万
• 依托单位: Muroran Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23510086/
• 关键词: テトラクロロエチレン; PCE; Propionibacterium; 脱塩素; DGGE; 環境浄化; 土壌浄化; PCEデハロゲナーゼ; 微生物群集構造解析

This research was performed to clarify the tetrachloroethylene (PCE) degradation mechanism using the new isolate, designated as strain Propionibacterium sp. HK-1. Groundwater and soil contamination by PCE are a global issue, and the creation of removal method is demanded socially. Strain HK-1 could dechlorinate not only PCE but also other various chlorinated aliphatic compounds. Groundwater and soil contaminations are combined contamination and the microbe which degrades various compounds is required for the implementation of bioremediation. Strain HK-1 has been considered to be one of candidates for the purpose and obtained academic results through this study are considered to be large on the application of PCE bioremediation.

本研究利用新分离菌株Propionibacterium sp. HK-1进行四氯乙烯（PCE）降解机理的研究。PCE对地下水和土壤的污染是一个全球性的问题，创造清除方法是社会需要。菌株HK-1不仅能脱氯PCE，还能脱氯其他各种氯化脂肪族化合物。地下水和土壤污染是复合污染，需要微生物降解各种化合物来实施生物修复。菌株HK-1被认为是候选菌株之一，通过本研究获得的学术成果被认为在PCE生物修复方面具有较大的应用价值。

项目成果

Biochemical and molecular characterization of a PCE-dechlorinating microorganism

四氯乙烯脱氯微生物的生化和分子表征

• 发表时间: 2012
• 期刊: Enzyme Engineering
• 作者: Young-Cheol Chang;Ken Sawada;Kazuhiro Takamizawa;and Shintaro Kikuchi
• 通讯作者: and Shintaro Kikuchi

Feasibility of kasugamycin production by Streptomyceskadugaensis using olive mill waste

卡杜加链霉菌利用橄榄厂废料生产春雷霉素的可行性

• 发表时间: 2011
• 期刊: J. Adv. Eng. and Tech.
• 作者: On-You Choi;Byeong-Kwon Ahn;Ki-An Cho;Gwang-Yeob Seo;In Sook Kang;and Young-Cheol Chang
• 通讯作者: and Young-Cheol Chang

Chitinase and alpha-amylase production from a lignin-degarading microorganism

木质素降解微生物生产几丁质酶和 α-淀粉酶

• 发表时间: 2013
• 作者: Young-Cheol Chang;Daiki Koyama;Ben Okeke;Shintaro Kikuchi
• 通讯作者: Shintaro Kikuchi

Characterization of salt and nitrate-tolerant PCB-utilizing bacteria

耐盐和硝酸盐 PCB 利用细菌的表征

• 发表时间: 2012
• 作者: Young-Cheol Chang;Ken Sawada;Kazunori Takada;Hoon Cho;Kikuchi Shintaro
• 通讯作者: Kikuchi Shintaro

Novosphingobium sp. TYA-1によるフェノール性内分泌撹乱化学物質の分解とその廃水処理への応用

Novosphingobium sp. TYA-1降解酚类内分泌干扰物及其在废水处理中的应用

• 发表时间: 2011
• 作者: 遠山 忠;開沼 侑子;チャン ヨンチョル;菊池 慎太郎;田中 靖浩;森 一博
• 通讯作者: 森 一博