Relatioship between functional gene and bacteria existed in Refuse-derived fuel and smoldering, fire and explosion

对废弃生物质燃料安全处理系统进行了基础研究；特别是防止与拒绝衍生燃料（RDF）有关的火灾和爆炸。RDF产氢能力受pH、湿度和环境温度的影响。微生物菌落计数法显示，在开始的第2天或第3天，RDF中需氧菌占优势，此后厌氧菌占优势。培养3 d后，Kuwana芽孢杆菌优于Clostridium， Sakura芽孢杆菌优于Bacillus。用三种不同量的RDF考察了生物活性引起的RDF温升的尺度效应。据报道，RDF用量越大，培养50 ~ 85 h后获得的最高温度越高。

The fundamental study for developing a safe handling system for waste biomass fuels was studied; especially for the prevention of fires and explosions associated with refused derive fuels (RDF). The RDF hydrogen production ability was affected by pH, moisture and ambient temperature. The microbial colony count method revealed that aerobes occupied a dominant in RDF on the 2nd or 3rd day from the beginning, whereas anaerobic occupied a dominant hereafter. Bacillus sp. was superior to Clostridium sp. in Kuwana, whereas Clostridium sp. was superior to Bacillus sp. in Sakura after 3 days of cultivation. Scale effects on the temperature rise of RDF due to biological activities were examined by three different amounts of RDFs. It was reported that the higher amount of RDF, the higher the maximum temperature obtained after 50 hours to 85 hours cultivation was.