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The biofilm formation mechanism and the chemical tolerance effect of the Legionella on the solid wall accompanied by a flow

军团菌在固体壁上伴随流动的生物膜形成机制及化学耐受效应

基本信息

批准号：
17500324
负责人：
KONISHI Tadashi
金额：
$ 2.11万
依托单位：
Oita National College of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

This study was aimed at proposing the infection preventive measures of legionellosis by the elucidation of the biofilm formation mechanism and the chemical tolerance effect of the legion Ella in artificial hydrological environment. In order to achieve the purpose of above which solves the biofilm formation mechanism in the piping intine accompanied by the flow which imitated actual warm water environment, and solves the defense mechanism over the drugs of a biofilm further, emphasis was put on the following two points. (1) Compare the biofilm formation mechanism in the solid wall of a static culture system with the biofilm formation mechanism on the solid wall of a flow medium system, and clarify a difference of the formation mechanism by temperature conditions and a flow condition. (2) Clarify existence of an appearance of the chemical effect (the chemical permeability into a biofilm, the survival ratio of Legion Ella, rate of recovery of a biofilm), and a chemical resistance bacteriu … More m to the biofilm formed on the solid wall in the flow medium system. Fruits of work are described below. The fluid type thermal gradient test equipment (Flow type Temperature Gradient Incubator: FTGI) and the Legionella culture apparatus which imitated actual warm water environment were manufactured, and the performance test was performed. After putting in and carrying out the aerobic culture of the Legionella of one agar medium cultivated to 10cm Petri-dish to a culture vessel for 48 hours, it cultivated by FTGI. Surface-of-a-wall temperature and fluid temperature were changed to 37 degrees and 42 degrees, respectively, sampled water was extracted every 24 hours, and the change in the number of legionella was measured by ATP measurement. Equipment was suspended about 48 hours afterward and biofilm adhesion of Legionella was observed to the surface-of-a-wall slide glass. The extracted biofilm was observed with BacLive/Dead dyeing and a fluorescence microscope, and the Legionella life-and-death status of the depth direction was observed by a confocal laser scanning microscope. The difference of the Legionella life-and-death in the temperature of 37 degrees and the biofilm by 42 degrees was clarified. Less

本研究旨在通过阐明军团菌在人工水文环境中生物膜形成机制和化学耐受效应，提出军团菌病的感染预防措施。为了达到上述目的，即解决模拟实际温水环境的流动伴随的管道内壁生物膜的形成机理，并进一步解决生物膜对药物的防御机理，本文重点研究了以下两点。(1)将静态培养系统固体壁面上的生物膜形成机理与流动介质系统固体壁面上的生物膜形成机理进行比较，并阐明温度条件和流动条件下生物膜形成机理的差异。(2)阐明化学效应的外观（化学品渗透到生物膜中，Legion Ella的存活率，生物膜的恢复率）和耐化学性细菌的存在。 ...更多信息 m至流动介质系统中固体壁上形成的生物膜。工作成果如下。制造模拟实际温水环境的流体型温度梯度试验设备（流动型温度梯度培养箱：FTGI）和军团菌培养装置，并进行性能试验。将培养至10cm皮氏平皿的一种琼脂培养基放入培养容器中进行军团菌需氧培养48小时后，用FTGI进行培养。将壁面温度和流体温度分别改变为37度和42度，每24小时抽取一次水样，通过ATP测量来测量军团菌数量的变化。约48小时后，将设备暂停，并观察到军团菌生物膜粘附到壁载玻片表面。采用BacLive/Dead染色法和荧光显微镜观察生物膜，激光共聚焦扫描显微镜观察军团菌在深度方向的生死状态。阐明了军团菌在37 ℃和42 ℃生物膜中的生死差异。少

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

STUDY ON RISK EVALUATION OF FLAMMABLE GAS GENERATING FROM WASTE BIOMASS

废弃生物质产生可燃气体的风险评估研究

DOI：
发表时间：
2006
期刊：
Thirty-first International Symposium on Combustion, abstracts of work-in progress poster presentations
影响因子：
0
作者：
T.Konishi;T;Yamashiro
通讯作者：
Yamashiro

Helicobacter pylori-associated oxidant monochloramine induces reactivation of Epstein Barr Birus in gastric epithelial cells latently infected with EBV.

幽门螺杆菌相关的氧化剂一氯胺诱导潜伏感染 EBV 的胃上皮细胞中 Epstein Barr Birus 的重新激活。

DOI：
发表时间：
2006
期刊：
Journal of Microbiology 55
影响因子：
0
作者：
Junko Minoura-Etoh;Kazuyo Gotoh;Ryugo Sato;Masao Ogata;Naomi Kaku;Toshio Fujioka;Akira Nishizono
通讯作者：
Akira Nishizono

固体壁上におけるレジオネラ属菌の不均質バイオフィルムの形成

在固体壁上形成军团菌异质生物膜