Development of in vitro toxicity methods for fire combustion products by using an artificial lung and tissue cells

建立了人肺泡基底上皮细胞A549对火焰气体毒性评价方法，作为大鼠、小鼠等动物实验的替代方法。燃气毒性评价系统由燃气收集设备、暴露设备和毒性评价设备组成。在ABS树脂实验中，初始细胞数；在5%- co_2环境下，3x10^5 cells/ml增加到3x10^6 cells/ml。在大气环境下，细胞增殖到1 × 10^6个/ml，存活率为90%。在火气环境下，细胞存活率降至5 × 10^4个/ml，存活率为20%。

The toxic valuation method of the fire gas by the human alveolus basal epithelium cells A549 was established as an alternative method of animal experiments, such as rat and mouse. The toxic assessment system of fire gas consists of fire gas collection equipment, exposure equipment, and toxic evaluation equipment. In the ABS resin experiment, the number of initial cells ; 3x10^5 cells/ml increased to 3x10^6 cells/ml after three-day fire gas exposure, and survival ratio was 95% under 5%-CO_2environment. It increased to 1x10^6 cells/ml and survival ratio was 90% under atmospheric environment. It decreased to 5x10^4 cells/ml and survival ratio became 20% under fire gas environment.