Creation of functional peptides recognizing a low-molecular compound by evolutionary molecular engineering

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We have succeeded to improve the preparation of cDNA display molecule easily and efficiently with a one-pot preparational manner. Furthermore, we demonstrated the biotin binding capacity of streptavidin magnetic beads decreased by mRNA-binding ribosomes, resulted in the low yield of cDNA display molecules. Secondly, we developed an easy and rapid molecular-protein interaction analysis using a cell-free translation and a novel puromycin-linker by a pull-down manner. Finally, by using these techniques, in vitro selection using 30 amino acids random library containing cysteine against amino group on the solid-phase was performed. As a result, an amino group-binding peptide aptamer containing two disulfide bonds which have a unique structure has explored. This shows that a peptide has capability to recognize small molecules with a low-molecular weight. In the future, peptides will be more and more applicable for nanotechnology as a recogniton material of nano sturcture.