Development of the automated faster acquisition technique for the observation of "the ultra-high voltage electron tomography"

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This study is intended to develop the automated faster acquisition technique for the observation of "the ultra-high voltage electron tomography", however, the observation needs much effort and spends lots of time in the usual way. Therefore, we developed the following items in the operation support system; (1) A new algorithm for automatic focus (AF), (2) an automatic correction of field of view, (3) a new high-speed imaging for the control of electron microscope, (4) an automated sequence control for acquiring images. As regards this particular AF algorithm, the technique which an optimized focus condition was determined from the image sharpness, was newly developed. We use two cameras dedicated to recording images and to controlling the electron microscope from images, whose cameras are optimized for each task. As a result, we accomplished the automation and reduction in time for the tomography observation.