Evaluation of the DNA Lesions Caused by Ionizing Radiation Using the Polymerase Chain Reaction

测量生物对电离辐射反应的生物剂量计对于辐射防护是有用的。我们报告了一个基于实时PCR方法的γ射线辐射剂量测定系统的开发和表征。实时PCR用于扩增和同时定量靶向DNA分子。如果由于限制性底物或试剂而没有限制，则在每个延伸步骤中，DNA靶的量加倍，导致特异性DNA片段的指数扩增。该检测的要点是，电离辐射引起的DNA损伤阻断了DNA聚合酶的DNA合成，导致与未受损DNA模板相比，受损DNA模板的扩增减少。

The biological dosimeter that measures biological responses to ionizing radiation is useful for radiation protection. We report the development and characterization of a gamma-ray irradiation dosimetry system based on Real-time PCR methodology. Real-time PCR is used to amplify and simultaneously quantify a targeted DNA molecule. If there are no limitations due to limiting substrates or reagents, at each extension step, the amount of DNA target is doubled, leading to exponential amplification of the specific DNA fragment. The essential point of this assay is that DNA lesions caused by ionizing radiation block DNA synthesis by DNA polymerase, resulting in a decrease in the amplification of a damaged DNA template compared with that of non-damaged DNA templates.