Evaluation of the DNA Lesions Caused by Ionizing Radiation Using the Polymerase Chain Reaction

使用聚合酶链式反应评估电离辐射引起的 DNA 损伤

• 批准号: 23561012
• 负责人: SHIMIZU Kikuo
• 金额: $ 3.49万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23561012/
• 关键词: DNA線量計; 個人被ばく線量; LET; リアルタイムPCR; 被ばく評価; 米国; 英国; PCR; バイオドシメトリ; DNA損傷; 吸収線量

The biological dosimeter that measures biological responses to ionizing radiation is useful for radiation protection. We report the development and characterization of a gamma-ray irradiation dosimetry system based on Real-time PCR methodology. Real-time PCR is used to amplify and simultaneously quantify a targeted DNA molecule. If there are no limitations due to limiting substrates or reagents, at each extension step, the amount of DNA target is doubled, leading to exponential amplification of the specific DNA fragment. The essential point of this assay is that DNA lesions caused by ionizing radiation block DNA synthesis by DNA polymerase, resulting in a decrease in the amplification of a damaged DNA template compared with that of non-damaged DNA templates.

测量生物对电离辐射的反应的生物剂量计对辐射防护是有用的。我们报道了一种基于实时荧光聚合酶链式反应方法的伽玛射线辐射剂量测量系统的开发和表征。实时聚合酶链式反应被用来扩增并同时定量目标DNA分子。如果没有限制底物或试剂的限制，在每个延伸步骤中，DNA靶标的数量都会加倍，导致特定DNA片段的指数级扩增。本实验的要点是，电离辐射引起的DNA损伤阻碍了DNA聚合酶的DNA合成，导致受损的DNA模板的扩增比未损伤的DNA模板的扩增减少。

项目成果

Fundamental Study of the Evaluation of the DNA Lesions Caused by High-LET Radiation exposure Using the Polymerase Chain Reaction

利用聚合酶链式反应评估高 LET 辐射引起的 DNA 损伤的基础研究

• 发表时间: 2012
• 作者: Youichirou Matuo;Yoshinobu Izumi;Kikuo Shimizu
• 通讯作者: Kikuo Shimizu

Study of the DNA damages caused by radiation exposure using the PCR : Enchancement with modified DNA template

使用 PCR 研究辐射暴露引起的 DNA 损伤：使用修饰的 DNA 模板进行增强

• 发表时间: 2013
• 作者: Ryuto Nakashima;Youichirou Matuo;Yuji Hidaka;Norihito Sato;Takayoshi Yamamoto;Kikuo Shimizu
• 通讯作者: Kikuo Shimizu

Evaluation of DNA lesions caused by high-LET radiation using the polymerase chain reaction reaction

使用聚合酶链式反应评估高 LET 辐射引起的 DNA 损伤

• 发表时间: 2013
• 期刊: Radiation Measurements
• 影响因子: 2
• 作者: Matuo;Yoshinobu Izumi;Norihito Sato;Takayoshi Yamamoto;Kikuo Shimizu
• 通讯作者: Kikuo Shimizu

Fundamental Study on molecular mechanism underlying repair of heavy-ion induced DNA damages in the Saccharomyces cerevisiae

重离子诱导的酿酒酵母DNA损伤修复分子机制的基础研究

• 发表时间: 2012
• 期刊: JAEA-Review
• 作者: Y.Matuo;Y. Izumi;Y.Hase;A.Sakamoto;S. Nozawa;I. Narumi and K. Shimizu
• 通讯作者: I. Narumi and K. Shimizu

Evaluation of the DNA Lesions Caused by High-LET Radiation Using the Polymerase Chain Reaction

使用聚合酶链式反应评估高 LET 辐射引起的 DNA 损伤

• 发表时间: 2011
• 作者: Youichirou Matuo;Yoshinobu Izumi;Norihito Sato;Takayoshi Yamamoto;Kikuo Shimizu
• 通讯作者: Kikuo Shimizu