Evaluation of the DNA Lesions Caused by Ionizing Radiation Using the Polymerase Chain Reaction

测量生物对电离辐射的反应的生物剂量计对辐射防护是有用的。我们报道了一种基于实时荧光聚合酶链式反应方法的伽玛射线辐射剂量测量系统的开发和表征。实时聚合酶链式反应被用来扩增并同时定量目标DNA分子。如果没有限制底物或试剂的限制，在每个延伸步骤中，DNA靶标的数量都会加倍，导致特定DNA片段的指数级扩增。本实验的要点是，电离辐射引起的DNA损伤阻碍了DNA聚合酶的DNA合成，导致受损的DNA模板的扩增比未损伤的DNA模板的扩增减少。

The biological dosimeter that measures biological responses to ionizing radiation is useful for radiation protection. We report the development and characterization of a gamma-ray irradiation dosimetry system based on Real-time PCR methodology. Real-time PCR is used to amplify and simultaneously quantify a targeted DNA molecule. If there are no limitations due to limiting substrates or reagents, at each extension step, the amount of DNA target is doubled, leading to exponential amplification of the specific DNA fragment. The essential point of this assay is that DNA lesions caused by ionizing radiation block DNA synthesis by DNA polymerase, resulting in a decrease in the amplification of a damaged DNA template compared with that of non-damaged DNA templates.