BIOLOGICAL EFFECTS OF ELECTROMAGNETIC FIELDS

电磁场的生物效应

• 批准号: 12831004
• 负责人: SHIMIZU Kikuo
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12831004/
• 关键词: yeast; DNA microarray; ELF; DNA chip; DNAチップ

There is widespread public concern over possible health effects of extremely low frequency magnetic fields (ELF-MF). We have previously investigated the biological effects of ELF-MF. Cell growth and mutagenesis have been studied with yeast as simple model system but no significant effects have been identified.In this study we used genome wide expression analysis to explore how gene expression in Sa cch a romyces cerevisiae is changed in response to exposure of ELF-MF.S.cerevisiase S288c was grown to the density 2 x 108 cells/ml and exposed with 0.5T, 60HzELF-MF for 3 hr. Total RNA wasisolated and cDNA was prepared from purified mRNA using reverse transcriptase.The cDNA was uniformly labeled using Cy3-dUTP (control) or Cy5-dUTP (exposed with ELF-MF). Both cDNAs-were mixed and hybridized to DNA microarray (yeast chip ver.1.1, DNA chip Research Inc. Japan) analyzed with ScanArray. The results demonstrate that more than ten genes out of 6400 yeast genes is enhanced to transcribe after ELF-MF exposure. Now we are analyzing those genes exactly.

人们普遍关注极低频磁场(ELF-MF)可能对健康造成的影响。我们之前已经研究了ELF-MF的生物学效应。在本研究中，我们利用全基因组表达分析的方法研究了酿酒酵母S288c在ELF-MF.S.cerevisiase S288c培养密度为2×108个/ml，并在0.5T，60HzELF-MF中暴露3小时后基因表达的变化。提取总RNA，经逆转录合成cDNA，用Cy3-dUTP(对照)或Cy5-dUTP(ELF-MF)统一标记。将这两个cDNA混合并杂交到DNA微阵列(酵母芯片1.1版，DNA芯片研究公司，日本)，用扫描阵列进行分析。结果表明，在6400个酵母基因中，有10多个基因在ELF-MF作用下得到了转录增强。现在我们正在对这些基因进行精确的分析。

项目成果

Nakaoka, Y. Takeda, R. & Shimizu, K.: "Orientation of Paramecium swimming in a DC magnetic field"Bioelectromagnetics. (in press). (2002)

中冈 Y. 武田 R.

清水喜久雄: "電磁界の細胞に及ぼす効果"ESI News. 18. 183-186 (2000)

Kikuo Shimizu：“电磁场对细胞的影响”ESI 新闻 18. 183-186 (2000)。

Nakaoka, Y: "Effect of a 60HZ Magnetic Field on Paramecium"Bioelectro magnetics. 21. 584-588 (2000)

Nakaoka, Y：“60HZ 磁场对草履虫的影响”生物电磁学。

Nakaoka, Y., & Shimizu, K., Hasegawa, K. and Yamamoto, T.: "Effect of a 60 Hz Magentic Field on the Behavior of Paramecium"Bioelectromagnetics. 21. 584-588 (2000)

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