Characterization of DNA polymerase φ from Saccharomyces cerevisiae

为了在体内分析DNA聚合酶φ的功能，构建了新的突变体（degron突变体）。POL5脱粒突变体（POL5 ^<td>）在DNA聚合酶φ基因上游含有泛素蛋白水解系统的底物肽。由于该突变体在高温（37度）下未过量产生UBC4基因产物，因此该突变体还具有降解体系中大量表达UBC4基因产物的质粒。利用DNA聚合酶φ突变体POL5 ^<td>对POL5基因进行了DNA芯片分析，研究了该基因在非允许温度下的表达规律，并与野生型在相同条件下的表达进行了比较。结果表明，没有获得特异性的突变基因簇。这说明POL5基因进行的是普遍的转录调控，而不是特定的基因群。

The new type mutant (degron mutant) was constructed in order to analyze the function of DNA polymerase φ in vivo. The degron mutant of POL5 (pol5^<td>) has the peptide which is substrate of proteolysis system of the ubiquitin in the upstream of the gene of DNA polymerase φ. This mutant also has plasmid for large manifesting UBC4 gene product of the degrading system, because it did not become the lethality in the high temperature (37 degrees) without overproducing the UBC4 gene product.The DNA microarray analysis of the POL5 gene was carried out in order to examine the pattern of the gene expression at non-permissive temperature using the mutant pol5^<td> of DNA polymerase φ, and it was compared with the expression of the wild type by same condition. The result showed that the specific gene cluster peculiarly changed was not obtained.This fact means that POL5 gene carries out universal transcriptional control not but specific gene group.