Characterization of DNA polymerase φ from Saccharomyces cerevisiae

为了分析DNA聚合酶φ的体内功能，构建了一种新型突变体（降解决定子突变体）。POL 5的降解决定子突变体（pol5^<td>）在DNA聚合酶φ基因上游具有作为泛蛋白水解系统底物的肽。该突变体也具有用于大量显示降解系统的UBC 4基因产物的质粒，因为它在高温（37 ℃）下没有过量产生UBC 4基因产物而没有成为致死性。为了使用DNA聚合酶φ的突变体pol 5 ^检查在非允许温度下的基因表达模式，进行了POL 5基因的DNA微阵列分析<td>，并将其与相同条件下野生型的表达进行了比较。结果表明，POL5基因没有得到特异性变化的基因簇，这说明POL5基因进行的不是普遍的转录调控，而是特定的基因组。

The new type mutant (degron mutant) was constructed in order to analyze the function of DNA polymerase φ in vivo. The degron mutant of POL5 (pol5^<td>) has the peptide which is substrate of proteolysis system of the ubiquitin in the upstream of the gene of DNA polymerase φ. This mutant also has plasmid for large manifesting UBC4 gene product of the degrading system, because it did not become the lethality in the high temperature (37 degrees) without overproducing the UBC4 gene product.The DNA microarray analysis of the POL5 gene was carried out in order to examine the pattern of the gene expression at non-permissive temperature using the mutant pol5^<td> of DNA polymerase φ, and it was compared with the expression of the wild type by same condition. The result showed that the specific gene cluster peculiarly changed was not obtained.This fact means that POL5 gene carries out universal transcriptional control not but specific gene group.