Analyses of ER-resident molecular chaperone-dependent nuclear membrane fusion during fertilization of Arabidopsis thaliana

我们发现，在拟南螺旋藻雌性配子体发生过程中，内质网Hsp70分子伴侣蛋白BiP参与了极核外、内核膜与不同j结构域伴侣蛋白的融合。实时成像分析表明，极核融合缺陷可在受精后第一次胚乳核分裂时恢复，受精时精子核融合是胚乳正常增殖所必需的。我们还鉴定了极核融合所需的候选核膜蛋白。

We showed that BiP, an Hsp70 molecular chaperone in the endoplasmic reticulum, function in the fusions of the outer and inner nuclear membranes of the polar nuclei with different sets of J-domain containing partner proteins during the female gametogenesis of A. thaliana. Live imaging analyses showed that the polar nuclei fusion defect can be recovered during the first endosperm nuclear division after fertilization and that the fusion of sperm nuclei at fertilization is required for proper endosperm proliferation. We also identified candidates of nuclear membrane proteins required for the polar nuclei fusion.