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Investigation into the LINE transposition process by structural study of DNA-protein complexes

Investigation into the LINE transposition process by structural study of DNA-protein complexes
通过 DNA-蛋白质复合物的结构研究来研究 LINE 转座过程
批准号:
23570139
负责人:
MAITA Nobuo
金额:
$3.49万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2013

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中文摘要
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英文摘要
The aim of this project is to reveal the structural basis of the LINE. To obtain the stable protein, I tested four EN-Myb constructs, but none could be expressed or purified better than Sart1-ORF2p. Next, I used Sf9 cells as protein expression system to overcome the codon-usage issue. Although I detected the ORF1p expression, ORF2p was not co-expressed. I tried to purify the ORF1p expressed in Sf9 cells, however, ORF1p was recovered in insoluble fraction and failed to purify.
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Crystallization strategy for the glycoprotein-receptor complex between measles virus hemagglutinin and its cellular receptor SLAM
麻疹病毒血凝素与其细胞受体SLAM之间糖蛋白受体复合物的结晶策略
DOI: 10.2174/092986612799789314
发表时间: 2012
期刊: Prot Pept Lett
影响因子: --
作者: [Hashiguchi T, Ose T, Kubota M, Maita N, Kamishikiryo J, Maenaka K, Yanagi Y.]
通讯作者: Yanagi Y.
マクロライド骨格をテンプレートとした新規キチナーゼ阻害剤の創製
以大环内酯骨架为模板创建新型几丁质酶抑制剂
DOI: --
发表时间: 2013
期刊:
影响因子: --
作者: [菅原章公, 他]
通讯作者:
Crystallization, X-ray diffraction analysis and SIRAS phasing of human α-L-iduronidase
人 α-L-艾杜糖醛酸酶的结晶、X 射线衍射分析和 SIRAS 定相
DOI: 10.1107/s1744309112040432
发表时间: 2012
期刊: Acta Crystallographica
影响因子: --
作者: [Maita N, Taniguchi H, and Sakuraba H]
通讯作者: and Sakuraba H
Crystallographic snapshots of Tom20-mitochondrial presequence interactions with disulfide-stabilized peptides
Tom20 线粒体前序列与二硫键稳定肽相互作用的晶体快照
DOI: 10.1021/bi200470x
发表时间: 2011
期刊: Biochemistry
影响因子: 2.9
作者: [Saitoh T, Igura M, Miyazaki Y, Ose T, Maita N, Kohda D.]
通讯作者: Kohda D.
14
    Development of protein crystallization tag using crystalline lattice
    • 批准号:
      15K13747
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.5万
    • 财政年份:
      2015
    • 负责人:
      MAITA Nobuo
    • 依托单位:
    Investigation of the DNA recognition mechanisms of sequence-specific LINE
    • 批准号:
      20770085
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $2.66万
    • 财政年份:
      2008
    • 负责人:
      MAITA Nobuo
    • 依托单位:
    海外基金