Investigation into the LINE transposition process by structural study of DNA-protein complexes
Investigation into the LINE transposition process by structural study of DNA-protein complexes
批准号:
23570139
负责人:
MAITA Nobuo
金额:
$3.49万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2013
中文摘要
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英文摘要
The aim of this project is to reveal the structural basis of the LINE. To obtain the stable protein, I tested four EN-Myb constructs, but none could be expressed or purified better than Sart1-ORF2p. Next, I used Sf9 cells as protein expression system to overcome the codon-usage issue. Although I detected the ORF1p expression, ORF2p was not co-expressed. I tried to purify the ORF1p expressed in Sf9 cells, however, ORF1p was recovered in insoluble fraction and failed to purify.
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Crystallization strategy for the glycoprotein-receptor complex between measles virus hemagglutinin and its cellular receptor SLAM
麻疹病毒血凝素与其细胞受体SLAM之间糖蛋白受体复合物的结晶策略
DOI:
10.2174/092986612799789314
发表时间:
2012
期刊:
Prot Pept Lett
影响因子:
--
作者:
[Hashiguchi T, Ose T, Kubota M, Maita N, Kamishikiryo J, Maenaka K, Yanagi Y.]
通讯作者:
Yanagi Y.
マクロライド骨格をテンプレートとした新規キチナーゼ阻害剤の創製
以大环内酯骨架为模板创建新型几丁质酶抑制剂
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[菅原章公, 他]
通讯作者:
他
Crystallization, X-ray diffraction analysis and SIRAS phasing of human α-L-iduronidase
人 α-L-艾杜糖醛酸酶的结晶、X 射线衍射分析和 SIRAS 定相
DOI:
10.1107/s1744309112040432
发表时间:
2012
期刊:
Acta Crystallographica
影响因子:
--
作者:
[Maita N, Taniguchi H, and Sakuraba H]
通讯作者:
and Sakuraba H
Crystallographic snapshots of Tom20-mitochondrial presequence interactions with disulfide-stabilized peptides
Tom20 线粒体前序列与二硫键稳定肽相互作用的晶体快照
DOI:
10.1021/bi200470x
发表时间:
2011
期刊:
Biochemistry
影响因子:
2.9
作者:
[Saitoh T, Igura M, Miyazaki Y, Ose T, Maita N, Kohda D.]
通讯作者:
Kohda D.
A novel function of the N-glycan revealed by the crystal structure of α-L-iduronidase
α-L-艾杜糖醛酸酶的晶体结构揭示了 N-聚糖的新功能
DOI:
--
发表时间:
2014
期刊:
影响因子:
--
作者:
[Saitoh T, Igura M, Miyazaki Y, Ose T, Maita N, Kohda D., Nobuo Maita]
通讯作者:
Nobuo Maita
共 14 条
Development of protein crystallization tag using crystalline lattice
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批准号:15K13747
-
项目类别:Grant-in-Aid for Challenging Exploratory Research
-
资助金额:$2.5万
-
财政年份:2015
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负责人:MAITA Nobuo
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依托单位:
Investigation of the DNA recognition mechanisms of sequence-specific LINE
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批准号:20770085
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.66万
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财政年份:2008
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负责人:MAITA Nobuo
-
依托单位:
海外基金