Investigation of the DNA recognition mechanisms of sequence-specific LINE

序列特异性 LINE 的 DNA 识别机制研究

• 批准号: 20770085
• 负责人: MAITA Nobuo
• 金额: $ 2.66万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770085/
• 关键词: レトロトランスポゾン; X線結晶構造解析

The final goal of this project was to obtain the crystal structure of LINE-EN : DNA to reveal how LINE-EN to bind to DNA and cleave it, but it was not completed. During the optimization for crystallization, I found that LINE-EN would form a dimer in solution, and that EN alone is not enough to make the stable complex with DNA and necessary a Myb-like domain located just downstream of EN.

这个项目的最终目标是获得Line-en的晶体结构：DNA，以揭示Line-en如何与DNA结合并切割它，但尚未完成。在结晶优化过程中，我发现LINE-EN会在溶液中形成二聚体，仅有EN不足以与DNA形成稳定的络合物，必须在EN下游有一个Myb样结构域。

项目成果

Global Conformational Change Associated with the Two-step Reaction Catalyzed by Escherichia coli Lipoate-Protein Ligase A

• DOI: 10.1074/jbc.m109.078717
• 发表时间: 2010-03-26
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Fujiwara, Kazuko;Maita, Nobuo;Taniguchi, Hisaaki
• 通讯作者: Taniguchi, Hisaaki

Comparative Structural Biology of Eubacterial and Archaeal Oligosaccharyltransferases

• DOI: 10.1074/jbc.m109.081752
• 发表时间: 2010-02-12
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Maita, Nobuo;Nyirenda, James;Kohda, Daisuke
• 通讯作者: Kohda, Daisuke

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Crystal structure of aminomethyltransferase in complex with dihydrolipoyl-H-protein of the glycine cleavage system

氨甲基转移酶与甘氨酸裂解系统的二氢硫辛酰-H-蛋白复合物的晶体结构

• 发表时间: 2010
• 期刊: Journal of Biological Chemistry
• 影响因子: 4.8
• 作者: Okamura-Ikeda;K.;et al.
• 通讯作者: et al.

Structure of the measles virus haemagglutinin bound to its cellular receptor SLAM

麻疹病毒血凝素与其细胞受体 SLAM 结合的结构

• 发表时间: 2011
• 期刊: Nature Structural & Molecular Biology
• 影响因子: 16.8
• 作者: Hashiguchi T.;et al.
• 通讯作者: et al.