Structure and assembly of the central hub of the baseplate of bacteriophage T4

噬菌体T4基板中心轮毂的结构与组装

• 批准号: 23570190
• 负责人: ARISAKA Fumio
• 金额: $ 3.49万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570190/
• 关键词: バクテリオファージ; 分子集合; 立体構造; 蛋白質間相互作用; 感染機構; 溶菌; 溶菌阻止; 尾繊維; テイルリゾチーム; 分子内シャペロン; ものさし蛋白質; T4リゾチーム; Ｔ４リゾチーム

1. Tube initiator protein, gp48, which were fused with SlyD at the N-terminus was expressed in a soluble fraction and crystallized. 2. The C-terminal domain of gp37 from phage PP01 which constitutes the tail fiber together with gp38 were co-expressed and the complex with 3:1 stoichiometry was isolated and crystallized. It was shown that the C-terminal 13 kDa polypeptide was self-cleaved. 3. The complex of the soluble domain of the holin and the rapid lysis protein gp rI was crystallized and the structure has been successfully determined, which is expected to contribute to understanding the mechanisma of lysis inhibition.

1。Tube引发剂蛋白GP48，在N末端与SLYD融合，以可溶性分数表达并结晶。 2。构成尾纤维与GP38的噬菌体PP01的GP37的C末端结构域共表达，并分离并与3：1化学计量学分离并结晶。结果表明，C末端13 kDa多肽是自裂的。 3。霍林的可溶性结构域的复合物和快速裂解蛋白GP RI结晶并成功地确定了结构，这有望有助于理解裂解抑制的机制。

项目成果

Genomic and proteomic characterization of the...

基因组和蛋白质组学特征...

• DOI: 10.4161/bact.1.3.16712
• 发表时间: 2011
• 期刊: Bacteriophage. Epub
• 作者: Tamakoshi M; Arisaka F;et al.
• 通讯作者: et al.

T4ファージのアンチホーリンによるホーリンの凝集抑制と溶菌阻止メカニズム

Antihorin抑制T4噬菌体聚集和溶菌裂解的机制

• 发表时间: 2011
• 作者: 有坂文雄;金丸周司;伊藤 悠太
• 通讯作者: 伊藤 悠太

Protein interactions in the assembly of the tail of bacteriophage T4

噬菌体 T4 尾部组装中的蛋白质相互作用

• 发表时间: 2013
• 期刊: Biophys Rev
• 作者: Munehito Arai;H. Jane Dyson;Peter E. Wright.;Ritsu Kamiya.;F. Arisaka and S. Kanamaru.
• 通讯作者: F. Arisaka and S. Kanamaru.

Stoichiometry of Protein Interactions in Bacteriophage T4 Tail Assembly in "Stoichiometry and Research --- The Importance of Quantity in Biomedicine" Edited by Alessio Innocenti

噬菌体 T4 尾部组装中蛋白质相互作用的化学计量，见 Alessio Innocenti 主编的《化学计量与研究 --- 数量在生物医学中的重要性》

• 发表时间: 2012
• 期刊: INTECH, Rijeka, Croatia
• 作者: H. Matsumoto;Y. Yamazaki;H. Kamikubo;M. Yamaguchi;M. Kataoka.;Fumio Arisaka.
• 通讯作者: Fumio Arisaka.

Redox and metal-regulated oligomeric state for...

氧化还原和金属调节的低聚状态...

• DOI: 10.1007/s00726-010-0570-y
• 发表时间: 2011
• 期刊: Amino Acids
• 影响因子: 3.5
• 作者: Sawada N;Nagahara N;Arisaka F;et al.
• 通讯作者: et al.