Constructing artificial rotors for rotary molecular motor F1(V1)-ATPase

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F1(V1)-ATPase is an ATP-driven rotary molecular motor. In view of what designed rotor can rotate, we have investigated which specific regions in the rotor contribute for the rotation. The mutant, in which only the below slight portion was left within contact regions between rotor axle and stator, rotated at half speed and generated half rotational torque relative to wild-type. Together with previous studies, no residues in the rotor are essential for the rotation. An essential property of the rotor may be just the stick-like structure, or any regions (residues) of rotor may contribute for the rotation.