Gemline development regulation by maternally localized factor PEM

母系局部因子 PEM 调节宝石发育

• 批准号: 23570252
• 负责人: KUMANO Gaku
• 金额: $ 3.41万
• 依托单位: Tohoku University (2013)Osaka University (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570252/
• 关键词: ホヤ; 生殖細胞; 転写抑制; 母性局在因子; PEM; 生殖系列; 初期発生; マボヤ

Suppression of zygotic transcription in early embryonic germline cells is tightly linked to its separation from the somatic lineage. Many invertebrate embryos utilize localized maternal factors that are successively inherited by the germline cells for silencing the germline. Germline quiescence has also been associated with the underphosphorylation of Ser2 of RNA polymerase II. Using an ascidian Halocynthia roretzi, we identified a first deuterostome example of a maternally localized factor, PEM, which represses germline transcription through Ser2 underphosphorylation. Our results suggest that non-homologous proteins, PEM, Pgc of Drosophila, and PIE-1 of C. elegans, repress germline gene expression through analogous functions: by keeping Ser2 underphosphorylated through binding to the P-TEFb complex, a kinase that phosphorylates Ser2. The present study provides an interesting example of evolutionary constraint on how a mechanism of germline silencing can evolve in diverse animals.

早期胚胎生殖系细胞合子转录的抑制与其从体细胞谱系中分离紧密相关。许多无脊椎动物胚胎利用由生殖系细胞连续遗传的局部母体因子来沉默生殖系。胚系静止也与RNA聚合酶II的Ser 2磷酸化不足有关。使用海鞘Halocynthia roretzi，我们确定了第一个后口的例子，母亲本地化的因素，PEM，通过Ser 2磷酸化不足抑制生殖系转录。我们的研究结果表明，果蝇的非同源蛋白PEM、Pgc和C. elegans通过类似的功能抑制生殖系基因表达：通过与P-TEFb复合物（一种磷酸化Ser 2的激酶）结合，保持Ser 2磷酸化不足。目前的研究提供了一个有趣的例子，进化的限制，如何生殖沉默的机制可以在不同的动物进化。

项目成果

The transcription factor FoxB mediates temporal loss of cellular competence for notochord induction in ascidian embryos

转录因子 FoxB 介导海鞘胚胎脊索诱导细胞能力的暂时丧失

• DOI: 10.1242/dev.070383
• 发表时间: 2011
• 期刊: Development
• 影响因子: 4.6
• 作者: Hashimoto;H.;Nishida;H.
• 通讯作者: H.

ホヤ幼生の尾が形づくられる仕組み

海鞘幼虫的尾巴是如何形成的

• 发表时间: 2013
• 作者: Hashimoto;H.;Nishida;H.;熊野岳;熊野岳
• 通讯作者: 熊野岳

Regulation of the number of cell division rounds by tissue-specific transcription factors and Cdk inhibitor during ascidian embryogenesis.

在西顺式胚胎发生过程中，通过组织特异性转录因子和CDK抑制剂来调节细胞分裂的数量。

• DOI: 10.1371/journal.pone.0090188
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kuwajima M;Kumano G;Nishida H
• 通讯作者: Nishida H

New Principles in Developmental Processes

• DOI: 10.1007/978-4-431-54634-4
• 发表时间: 2014
• 作者: H. Kondoh;A. Kuroiwa

Transcription factor Tbx6 plays a central role in fate determination between mesenchyme and muscle in embryos of the ascidian, Halocynthia roretzi.

转录因子 Tbx6 在海鞘 (Halocynthia roretzi) 胚胎中间充质和肌肉之间的命运决定中发挥着核心作用。

• DOI: 10.1111/dgd.12133
• 发表时间: 2014
• 期刊: Dev. Growth Differ.
• 作者: Kumano;G.;Negoro;N.;and Nishida;H.
• 通讯作者: H.