Cellular protein quality control mechanism and the evolution of chemokine CXCL1L gene

食蟹猴具有最近通过基因复制产生的趋化因子基因CXCL1和CXCL1L，而其他哺乳动物如人类仅具有CXCL1，并且CXCL1L在这些物种中失活。然而，食蟹猴的CXCL1L被转录，但mRNA几乎不翻译成分泌蛋白。为了阐明CXCL1L蛋白在食蟹猴中表达抑制的机制，我们因此研究了细胞蛋白质质量控制机制参与蛋白质表达抑制的可能性。虽然我们发现，与CXCL1 mRNA相比，与CXCL1L mRNA结合的核糖体数量减少，但结果表明，蛋白质质量控制机制不参与蛋白质表达的抑制。

Cynomolgus monkeys have chemokine genes CXCL1 and CXCL1L generated recently by gene duplication, whereas other mammals such as humans have only CXCL1, and the CXCL1L is inactivated in those species. However, the CXCL1L of cynomolgus monkeys is transcribed but the mRNA is scarcely translated into a secreted protein. To ellucidate the mechanism of the suppression of the CXCL1L protein expression in cynomolgus monkeys, we therefore investigated the possibility of an involvement of cellular protein quality control mechanism in the suppression of the protein expression. Although we found that the number of ribosomes bound to the CXCl1L mRNA is reduced compared to the CXCL1 mRNA, the results show that the protein quality control mechanism is not involved in the suppression of protein expression.