Cellular protein quality control mechanism and the evolution of chemokine CXCL1L gene

细胞蛋白质量控制机制及趋化因子CXCL1L基因的进化

• 批准号: 23570275
• 负责人: NOMIYAMA Hisayuki
• 金额: $ 3.49万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570275/
• 关键词: ケモカイン; 遺伝子進化; アカゲザル; タンパク質発現; 翻訳; タンパク質発現抑制

Cynomolgus monkeys have chemokine genes CXCL1 and CXCL1L generated recently by gene duplication, whereas other mammals such as humans have only CXCL1, and the CXCL1L is inactivated in those species. However, the CXCL1L of cynomolgus monkeys is transcribed but the mRNA is scarcely translated into a secreted protein. To ellucidate the mechanism of the suppression of the CXCL1L protein expression in cynomolgus monkeys, we therefore investigated the possibility of an involvement of cellular protein quality control mechanism in the suppression of the protein expression. Although we found that the number of ribosomes bound to the CXCl1L mRNA is reduced compared to the CXCL1 mRNA, the results show that the protein quality control mechanism is not involved in the suppression of protein expression.

食蟹猴的趋化因子基因CXCL1和CXCL1L是最近通过基因复制产生的，而其他哺乳动物，如人类，只有CXCL1，而CXCL1L在这些物种中是失活的。然而，食蟹猴的CXCL1L是转录的，但其mRNA几乎不能翻译成分泌蛋白。因此，为了阐明食蟹猴CXCL1L蛋白表达抑制的机制，我们研究了细胞蛋白质量控制机制参与抑制该蛋白表达的可能性。虽然我们发现与CXCL1L mRNA结合的核糖体数量比CXCL1mRNA少，但结果表明蛋白质质量控制机制不参与蛋白质表达的抑制。

项目成果

脊椎動物ケモカインシステムの進化における変遷 Evolutionary history of the vertebrate chemokine system

脊椎动物趋化因子系统的进化史

• 发表时间: 2011
• 作者: 野見山尚之、長田直樹、義江修 Nomiyama H;Osada N and Yoshie 0
• 通讯作者: Osada N and Yoshie 0

Systematic classification of vertebrate chemokines based on conserved synteny and evolutionary history.

• DOI: 10.1111/gtc.12013
• 发表时间: 2013-01
• 期刊: Genes to cells : devoted to molecular & cellular mechanisms
• 作者: Nomiyama H;Osada N;Yoshie O
• 通讯作者: Yoshie O

CXCL5.

CXCL5。

• 发表时间: 2012
• 期刊: 臨床免疫・アレルギー科
• 作者: Francoise Bachelerie;Adit Ben-Baruch;Amanda M. Burkhardt;Christophe Combadiere;Joshua M. Farber;Gerard J. Graham;Richard Horuk;Alexander Hovard Sparre-Ulrich;Massimo Locati;Andrew D. Luster;Alberto Mantovani;Kouji Matsushima;Philip M. Murphy;Hisayuki Nomiyama et al.;野見山尚之,棚瀬純男
• 通讯作者: 野見山尚之,棚瀬純男

齧歯類および重歯類ケモカインのゲノム多様化機構Diversification Mechanism of Rodent and Lagomorpha Chemokine Genes

啮齿动物和兔类趋化因子基因的多样化机制

• 发表时间: 2012
• 作者: 柴田佳菜子、野見山尚之、棚瀬純男 Shibata K;Nomiyama H and Yoshi O
• 通讯作者: Nomiyama H and Yoshi O

Genome diversification mechanism of rodent and Lagomorpha chemokine genes.

• DOI: 10.1155/2013/856265
• 发表时间: 2013
• 期刊: BioMed research international
• 作者: Shibata K;Nomiyama H;Yoshie O;Tanase S
• 通讯作者: Tanase S