Comparison of functions on intestinal microbes between strains of Bifidobacterium and Ruminococcus.

不易消化的糖类对控制结肠微生物群很重要。果寡糖（Fructo-oligosaccharide， FOS）是一种典型的难消化低聚糖，能增加双歧杆菌的细胞。利用微生物酶有效地制备了不同难消化低聚糖DFAIII（二果糖酸酐III）。DFAIII对产瘤球菌AHU1760的生长有促进作用，但对双歧杆菌的生长没有促进作用，因为双歧杆菌不能降解DFAIII。在本研究中，我们比较了DFAIII +产瘤球菌AHU1760与FOS +短双歧杆菌JCM1192T对健康的影响。这两种组合都是共生的。以富含FOS的天然蔬菜雪莲果与FOS试剂进行比较。选用5周龄SD雄性大鼠，分别饲喂正常饲料和高脂饲料。饲养30 d，采用高效液相色谱法、DNA PCR-DGGE分析盲肠pH、有机酸组成、微生物群。这两种共生菌的盲肠内容物和粪便pH值均明显降低，表明肠道微生物大量生长，产生了丰富的发酵。在高脂肪条件下观察到差异。DFAIII与产瘤球菌AHU1760共生，使肠道发酵持续丰富，保持较低的肠道pH，而FOS与短双歧杆菌JCM1192T不共生。这些结果得到DNA PCR-DGGE分析的支持。产瘤球菌AHU1760的保健效果优于短双歧杆菌JCM1192T。雏鸡死亡后肠道菌群和pH值变化较快，健康效果优于FOS试剂。

In-digestible saccharides are important to control microbiota in colon. Fructo-oligosaccharide (FOS) is a typical in-digestible oligosaccharide, and it increase cells of Bifidobacterium species. We made process to make different in-digestible oligosaccharide which is DFAIII (di-fructose anhydride III) with using microbial enzyme effectively. DFAIII enhanced growth of Ruminococcus productus AHU1760 which was isolated from human feces, but not growth of Bifidobacterium strains because Bifidobacterium strains can’t degrade DFAIII. In this study we compared health effect of DFAIII + Ruminococcus productus AHU1760 vs. FOS + Bifidobacterium breve JCM1192T. Both combination were symbiotic. Yacon which is FOS rich natural vegetable were used as comparison with reagent FOS. Rat used were SD male 5 weeks with normal feed and high fat feed were used. Rats were kept for 30 days and analyzed pH, organic acid compositions, microbiota in cecum with using HPLC, DNA PCR-DGGE.Both symbiotic showed clear pH decrease in cecal contents and feces which indicated abundant intestinal fermentation by abundant growth of microbes. Differences were observed in high fat conditions. Symbiotic by DFAIII and Ruminococcus productus AHU1760 continued abundant intestinal fermentations and kept intestinal pH in lower condition but not symbiotic by FOS and Bifidobacterium breve JCM1192T. Those results were supported DNA PCR-DGGE analysis. Ruminococcus productus AHU1760 showed better health effect than Bifidobacterium breve JCM1192T. Yacon died gave faster change of intestinal microbiota and pH which shoeing better health effect than reagent FOS.